天然产物研究与开发2017，Vol.29Issue(11)：1940-1945,6.DOI:10.16333/j.1001-6880.2017.11.021
川芎油对氯化钻损伤PC12细胞保护作用及其活性成分研究
Protective Effects of Ligusticum chuanxiong Oil on PC12 Cells Injured by CoCl2 and Its Active Components
摘要
Abstract
The chemical constituents of the essential oil from Ligusticum chuanxiong were separated and purified by various chromatographic techniques including silica gel and Sephadex LH-20 column chromatography,normal phase (NP) semi-preparative HPLC and reversed phase (RP) semi-preparative HPLC.Their structures were identified by physicochemical properties,spectroscopic analysis,as well as comparison with the literature data.PC12 cells injured by CoCl2 were used as the cell model of hypoxia.The viability and lactate dehydrogenase(LDH) release of PC12 cells were separately detected by MTT assays and LDH detecting kits,and the apoptotic cells were stained by Hoechst 33258.Six compounds were isolated from the essential oil of L.chuanxiong and identified asriligustilide (1),endo-Z,Z'-(3a.7a',7a.3a')-diligustilide (2),spathulenol (3),(E)-3-butylidenephthalide (4),3-butylidene-7-hydroxyphthalide (5),and senkyunolide I(6).It was proved that the oil and compound 1 (riligustilide) protected the injury and inhibit the apoptosis induced by CoCl2 in PC12 cells,and reduced the level of LDH in cells.These results demonstrated that the essential oil of L.chuanxiong and compound 1 (riligustilide) had protective effect for injured neurons in appropriate concentration range,and provided scientific reference for pharmacodynamic material basis of L.chuanxiong.关键词
川芎/挥发油/化学成分/PC12细胞保护Key words
Ligusticum chuanxiong/essential oil/chemical constituents/protective effect on PC12 cells分类
医药卫生引用本文复制引用
王月,刘露丝,刘娟,彭成,熊亮..川芎油对氯化钻损伤PC12细胞保护作用及其活性成分研究[J].天然产物研究与开发,2017,29(11):1940-1945,6.基金项目
四川省科技支撑计划川芎整合式全产业链综合开发研究(2015SZ0031) （2015SZ0031）
科技基础性工作专项计划(2015FY111500-140) （2015FY111500-140）
四川省青年科技创新研究团队专项计划(2017TD0001) （2017TD0001）
