现代妇产科进展2017，Vol.26Issue(12)：881-884,4.DOI:10.13283/j.cnki.xdfckjz.2017.12.001
青蒿素衍生物对宫颈癌HeLa细胞体外增殖及凋亡能力的影响及其分子机制研究
Analysis of the effects of artemisinin derivatives on proliferation and apoptosis of HeLa cells in cervical cancer and its molecular mechanism.
摘要
Abstract
Objective:To investigate the effect and the molecular mechanism of arte-misinin derivatives(dihydroartemisinin and artesunate,DHA and ART)on the proliferation、ap-optosis of cervical cancer cells(HeLa)in vitro. Methods:Cervical cancer cells were exposed to indicated concentrations of DHA,ART and DDP for different hours,and the MTT assay was used to observe the effects of DHA,ART and DDP on the proliferation of cervical cancer cells in vitro. And the flow cytometry was used to verify their effect on the apoptosis of cervical cancer cells in vitro. Then the phosphorylation level of MAPKs of cervical cancer cells was detected by Western blot. Results:The IC 50 of HeLa cell lines by DHA and ART for 48h were (8. 9±1. 1)μmol/ L and (10. 5 ±1. 2)μmol/ L respectively. Compared with the control group,DHA and ART reduced the phosphorylated ERK1 / 2 levels of HeLa by (70. 5 ±2. 6)%(P<0. 05)and (65. 8 ±2. 9)% (P <0. 05),increased the phosphorylated p38 levels of HeLa by (57. 3 ± 2. 4)%(P<0. 05)and (47. 0±3. 0)%(P<0. 05).DHA and ART can promote the apoptosis of HeLa in vitro. DHA and ART increased the apoptosis of HeLa after treatment with 12h to (51. 0±3. 6)%(P<0. 05)and (38. 4 ±3. 2)%(P<0. 05)respectively. Conclusion:For the cervical cancer cell lines,DHA and ART can inhibit their growth-ability and promote the apop-tosis of HeLa in vitro. It might be related to the down-regulating phosphorylation level of ERK1 /2,and up-regulating phosphorylation level of p38.关键词
青蒿素衍生物/宫颈癌/体外增殖/凋亡Key words
Artemisinin derivatives/Cervical cancer/Proliferation in vitro/Apoptosis分类
医药卫生引用本文复制引用
杨华,谭先杰,郎景和,吕江涛..青蒿素衍生物对宫颈癌HeLa细胞体外增殖及凋亡能力的影响及其分子机制研究[J].现代妇产科进展,2017,26(12):881-884,4.基金项目
国家自然科学基金项目资助(No:30973177) （No:30973177）
