现代妇产科进展2017，Vol.26Issue(12)：893-897,5.DOI:10.13283/j.cnki.xdfckjz.2017.12.003
绵羊肌源性干细胞负载非交联生物补片植入绵羊阴道缺损模型的组织学和在体生物力学研究
Histological and in vivo biomechanical studies on sheep vaginal defect models repaired with non-cross linked biological patches loaded with sheep muscle-derived stem cells. Li
摘要
Abstract
Objective:To establish the sheep model of the vaginal defects and implan-tation of porcine biological patches integrated with sheep muscle-derived stem cell(MDSC)and to understand the feasibility of transplantation of heterogeneous porcine biological patches with MDSC to regenerate vaginal tissue. Methods:The MDSC was seeded on the surface of non-crosslinked porcine biological patches from acellular porcine pericardium matrix and small in-testinal submucosa matrix. The sheep model of the vaginal defect and implantation of heteroge-neous biological patch integrated with sheep MDSC were established. The expression of a-SMA were detected by immunohistochemistry,at 1 week,1 months and 3 months. The biomechanical characteristics of the regenerative tissue were detected by biomechanical testing when the pat-ches implantation for 3 months. Results:The sheep MDSC grew well on the surface of porcine biological patch. Biological patches integrated with MDSC implant group had a better histocom-patibility. The a-SMA expressions in biological patches integrated with MDSC implant group were greater. The degeneration speeds of non-cross-linked S-type biological patches were slower and their tensile properties were better than other groups for 3 months. The ultimate tensile strength of them was (20. 38±2. 45)N/ mm2 ,and they still had good biomechanical properties. Conclusions:The sheep can be a good model for human vaginal exploratory research. Non-cross-linked S-type patches integrated with MDSC can promote smooth muscle regeneration, which can provide a good biomechanical support.关键词
肌源性干细胞/盆腔器官脱垂/动物模型/组织工程Key words
Muscle-derived stem cells/Pelvic organ prolapse/Animal model/Tissue engineering分类
医药卫生引用本文复制引用
李玢,陈俏华,沈鑫,姜向阳,邓卓,朱兰..绵羊肌源性干细胞负载非交联生物补片植入绵羊阴道缺损模型的组织学和在体生物力学研究[J].现代妇产科进展,2017,26(12):893-897,5.基金项目
国家自然科学基金青年项目(No:81501238) （No:81501238）
陕西省科技厅社会发展计划项目(No:2013K12-11-02) （No:2013K12-11-02）
