现代妇产科进展2017，Vol.26Issue(12)：901-904,4.DOI:10.13283/j.cnki.xdfckjz.2017.12.005
上皮性卵巢癌转移模式相关分子初探
The study of molecular markers associated with clinical classification of epithelial ovarian cancer.
摘要
Abstract
Objective:Screening different moleculars in tumor tissue from patients with ovarian cancer who presented peritoneal dissemination and / or lymph node metastasis. Meth-ods:Detecting different moleculars in tumor tissue from ovarian cancer patients (5 patients in the lymph node metastasis group while 4 patients in the peritoneal metastasis group)with hu-man tumor metastasis PCR array. Then confirming the identified moleculars in tumor tissue with western blot. Finally,confirming the moleculars in paraffin-embedded ovarian cancer tissue with immunohistochemistry assay. Results:Fourteen molecules were found to be significantly differ-ent for mRNA between the two groups. SMAD4,CCL7,CDKN2A and SERPINE1 were chosen according to the published reports in ovarian cancer and other malignancies for further study for they had been reported to be correlated with either lymph node metastasis or peritoneal metasta-sis in other malignancies. In the grey level difference analysis of Western blot,CCL7,CDKN2A (P16INK4a)and SERPINE1 showed higher level of protein expression in specimens of Group I than Group II(P<0. 05). In IHC assay,analysis indicated that only PAI-1 protein showed sig-nificant different expression levels in lymph node metastasis group,peritoneal dissemination group and mixed group,the average score were 10. 7,7. 28 and 8. 13 respetively (P =0. 036). Conclusion:PAI-1 protein may be one of the moleculars with different expression level in epi-thelial ovarian cancer with different metastasis modes,and it is expected to become a marker for molecular classification of ovarian cancer.关键词
卵巢癌,上皮性/转移,淋巴结/转移,腹膜/分型/分子标志物Key words
Ovarian cancer,Epithelial/Metastasis,Lymph node/Peritoneal dissemina-tion/Classification/Molecular marker分类
医药卫生引用本文复制引用
黄绮丹,冯艳玲,顾海风,刘继红..上皮性卵巢癌转移模式相关分子初探[J].现代妇产科进展,2017,26(12):901-904,4.基金项目
国家高技术研究发展计划(863计划)(No:2012AA02A507) （863计划）
