现代妇产科进展2017，Vol.26Issue(12)：911-916,6.DOI:10.13283/j.cnki.xdfckjz.2017.12.008
循环miRNA-93在DHEA多囊卵巢综合征大鼠模型中的表达及其意义
The study on the expression and diagnostic value of circulating miRNA-93 in a DHEArat PCOS model.
摘要
Abstract
Objective:Through detecting the expressions of miRNA-93 and vascular endothelial growth factor A (VEGFA)in serum and ovary tissue of rats with PCOS,to explore the mechanism and diagnostic value of circulating miRNA-93 and target gene VEGFA in the de-velopment and progression of PCOS. Methods:3 ~ 4 week old female (SD)rats were randomly average divided into control group and experimental group. The experimental group received DHEA 6mg / 100g weight and the control group received 0. 2ml injection oil respectively by sub-cutaneous injection on neck and back daily. After 28 days,the estrous cycle,ovarian morpholo-gysex hormone,body weight,ovarian volume,OGTT blood glucose,fasting insulin,insulin (HO-MA-IR)changes and lipid metabolism of the two groups of rats were tested. The miRNA-93 in serum and ovarian tissue was detected by real-time quantitative PCR (RT-PCR),and the VEG-FA in serum was detected by enzyme linked immunosorbent assay (ELISA). The VEGFA in o-varian tissue was detected by immunohistochemistry (IHC)and Western blot. Results:Themodel rat groups were the regular estrous cycle lost,ovarian volume enlarged and polycystic,se-rum FSH,T and LH increased,glucose tolerance abnormal. The fasting insulin and insulin re-sistance index was significantly higher than the control group. Meanwhile the serum cholesterol and triglyceride were higher than the control group. The model of PCOS rats was successfully constructed. RT-PCR,ELISA,Western blot,IHC and other assay results showed that the expres-sions of miRNA-93 and target gene VEGFA in the serum and ovary tissues of PCOS rats in-creased significantly (P<0. 05). Conclusions:The miRNA-93 in serum and ovary tissues of P-COS rats was highly expressed and may play an important role in the development of PCOS by regulating its target gene VEGFA. Circulating miRNA-93 may represent a novel non-invasive di-agnostic for PCOS.关键词
多囊卵巢综合征(PCOS)/脱氢表雄酮(DHEA)/循环miRNA-93/血管内皮生长因子A(VEGFA)/诊断标记物Key words
Polycystic ovary syndrome (PCOS)/Dehydroepiandrosterone (DHEA)/Circulating miRNA-93/Vascular endothelial growth factor A (VEGFA)/Diagnostic markers分类
医药卫生引用本文复制引用
邢莹莹,李青春,韩艳艳,李小琳,田晓予..循环miRNA-93在DHEA多囊卵巢综合征大鼠模型中的表达及其意义[J].现代妇产科进展,2017,26(12):911-916,6.基金项目
河南省卫计委科技攻关项目(No:201503140) （No:201503140）
