眼科新进展2017，Vol.37Issue(12)：1110-1113,1118,5.DOI:10.13389/j.cnki.rao.2017.0280
脑源性神经营养因子对糖尿病大鼠视网膜Müller细胞的保护作用
Effect of brain-derived neurotrophic factor on retinal Müller cells in diabetic rats
摘要
Abstract
Objective To investigate the protective effect of brain derived neurotrophic factor (BDNF) on Müller cells in the retina of diabetic rats.Methods A total of 54 healthy male SD rats were recruited and randomly divided into control group,diabetic group and BDNF group.Then a diabetic model was established by intraperitoneal injection of streptozotocin in rats of diabetic and BDNF groups.Preparation of BDNF injection was performed using PBS balanced salt solution containing 0.1 g · L 1 BSA.The BDNF group was given BDNF injection,while the control and diabetic group were injected with equal dose of PBS balanced salt solution 4 weeks after successful modeling.And after 8 weeks,the expression of L-glutamate/L-aspartate transporter (GLAST),glutamine synthetase (GS) and synaptophysin (SYN) were detected by immunofluorescence technique and Western blot,and the content of glutamic acid in retina was determined by glutamic acid determination kit.Results Compared with the control group,the expression of GLAST,GS and SYN were significantly decreased in diabetic group,and the content of glutamic acid was increased significantly (all P ＜ 0.01).Compared with the diabetic group,the expression of GLAST,GS and SYN were increased in BDNF group,and the glutamate level was decreased significantly (all P ＜0.01).Conclusion In the early stage of diabetic retinopathy,administration of exogenous BDNF can unregulated the expression of GLAST,SYN and GS and improve the function of Müller cells to protect RGC against damage,suggesting that BDNF has neuroprotective effects on Müller cells in retina of rats with diabetic retinopathy.关键词
脑源性神经营养因子/糖尿病视网膜病变/Müller细胞/L-谷氨酸/L-天冬氨酸转运体Key words
brain-derived neurotrophic factor/diabetic retinopathy/Müller cells/L-glutamate/L-aspartate transporter分类
医药卫生引用本文复制引用
梁汇珉,李赵伟,李铮,左中夫,刘学政..脑源性神经营养因子对糖尿病大鼠视网膜Müller细胞的保护作用[J].眼科新进展,2017,37(12):1110-1113,1118,5.基金项目
国家自然科学基金资助(编号:81571383)National Natural Science Foundation of China (No:81571383) （编号:81571383）
