眼科新进展2017，Vol.37Issue(12)：1114-1118,5.DOI:10.13389/j.cnki.rao.2017.0281
维生素B12对糖尿病大鼠视网膜神经细胞凋亡的影响
Effects of vitamin B12 on apoptosis of retinal ganglion cells of diabetic rat
摘要
Abstract
Objective To investigate the effects of vitamin B12 on apoptosis in retinal ganglion cells of rat with diabetes and its underlying mechanisms.Methods Together 36 clean SD rats were randomly divided into 3 groups (n =12):negative contxol (NC) group,diabetic mellitus (DM) group and vitamin B12 treatment (VitB12)group.Then a model diabetic rats was conducted by intraperitoneal administration of streptozotocin in rats of DM and VitB12 groups.After modeling successfully,100 μg ·kg-1 vitamin B12 was given to rats in the VitB12 group by intragastric administration 1 time per day for 8 consecutive weeks,and an equivalent amount of normal saline was utilized for the animals of NC and DM groups in the same way.After 10 weeks,all the rats were sacrificed to collect jugular blood for detection of serum malondialdehyde (MDA) level and superoxide dismutase (SOD) activity;and the retinal tissue was also collected for preparation of paraffin sections for observing the apoptosis of retinal ganglion cells and counting the apoptotic index (AI) by TUNEL staining.Finally,the expression of Caspase-3 in the retina was detected by immunohistochemistry.ResultsThe levels of serum MDA in the DM and VitB12 groups were both higher than that in the NC group (all P ＜ 0.01),and DM group was higher than VitB12 group (P ＜ 0.01);and there was significant difference among the three groups (F =471.061,P =0.000).The activity of SOD in the DM and VitB12 groups were both lower than that in the NC group (all P ＜0.01),and DM group was lower than VitB12 group (P ＜ 0.01);and there was significant difference in the three groups (F =356.923,P =0.000).Apoptotic cells were observed in retinal ganglion cell layer,inner and outer nuclear layer in three groups,and the number of apoptotic cells in the DM and VitB12 groups was significantly larger than that in the NC group,but the number of apoptotic cells in VitB12 group was decreased to some extent compared with DM group.The AI of retinal ganglion cells in the DM and VitB12 groups were both higher than that in the NC group (all P ＜ 0.01),and DM group was higher than VitB12 group (P ＜ 0.05);and there was significant difference among the three groups (F =14.926,P =0.000).Microscopic examination showed Caspase-3 protein expression was appeared in the retinal ganglion cell layer,the inner and the outer nuclear layer in the three groups.The Caspase-3 protein expression in DM and VitB12 groups was significantly increased compared with NC group,and VitB12 group was significantly lower than DM group.Moreover,the optical density of Caspase-3 in the DM and VitB12 groups was higher than that in the NC group (all P ＜0.01),and DM group was higher than VitBl 2 group (P ＜ 0.01),and there was significant difference among the three groups (F =77.896,P =0.000).Conclusion Vitamin B12 has certain protective effect on retinal ganglion cell of diabetic rats,of which the mechanism may be its antioxidant stress can inhibit the expression of Caspase-3,thereby suppressing the apoptosis of retinal ganglion cells.关键词
维生素B12/糖尿病视网膜病变/氧化应激/Caspase-3/凋亡Key words
vitamin B12/diabetic retinopathy/oxidative stress/Caspase-3/apoptosis分类
医药卫生引用本文复制引用
徐卫星,李勇,徐军..维生素B12对糖尿病大鼠视网膜神经细胞凋亡的影响[J].眼科新进展,2017,37(12):1114-1118,5.基金项目
辽宁省科技厅联合基金项目(编号:2015020351)Joint Fund Project of Liaoning Provincial Science and Technology Department (No:2015020351) （编号:2015020351）
