眼科新进展2017，Vol.37Issue(12)：1132-1134,3.DOI:10.13389/j.cnki.rao.2017.0285
无血清条件下组织块法培养人角膜上皮细胞的比较
Comparison of explant culture methods for human corneal epithelial cell with free-serum
史小玲 1王骞 1鹿晓燕2
作者信息
- 1. 361001 福建省厦门市,厦门大学附属厦门眼科中心
- 2. 450052 河南省郑州市,郑州大学第一附属医院眼科
- 折叠
摘要
Abstract
Objective To compare and analyze the time of cell eruption formation and the rate of cell eruption of human corneal epithelial cells cultured by different explant culture methods under serum-free conditions.Methods Explant tissues were randomly divided into A,B,C and D groups.In A group,the cornea epithelial cells were put side up after stripping corneal endothelium and scraping the corneal epithelium;in B group,the corneal epithelium was put side down after stripping corneal endothelium and scraping the corneal epithelium;in C group,the corneal epithelium was put side up after only stripping corneal endothelium;and in D group,the corneal epithelium was put side down after only stripping corneal endothelium.The morphological changes of the cells in the 4 groups were observed by phase-contrast microscope,and both aforementioned variable were recorded.Results The average time of cell eruption formation and the rate of cell eruption in A,B,C and D group was (7.00 ± 3.00) d and 47.50％ (19/40),(7.55 ±2.58)d and 45.83％ (11/24),(8.43 ±3.32)d and 63.64％ (14/22),(7.49 ± 2.20) d and 72.22％ (39/54),respectively.The chi-square test showed that there was significant difference in the cell eruption rate between A and D group as well as B and D group (all P ＜ 0.05),but there was no significant difference between the other groups (all P ＞ O.05).Conclusion The time of cell eruption formation in the four different explant culture methods was about one week in free-serum conditions.And D group has higher cell eruption rate,and pure plus stable cultured cells when compared with the other three groups.关键词
角膜上皮细胞/组织块法/无血清/细胞培养Key words
corneal epithelial cell/ explant culture/serum-free/ cell culture分类
医药卫生引用本文复制引用
史小玲,王骞,鹿晓燕..无血清条件下组织块法培养人角膜上皮细胞的比较[J].眼科新进展,2017,37(12):1132-1134,3.
