应用生态学报2017，Vol.28Issue(11)：3479-3486,8.DOI:10.13287/j.1001-9332.201711.020
大气臭氧浓度升高对银杏叶片活性氧代谢及相关基因表达的影响
Effects of elevated ozone concentrations on reactive oxygen metabolism and related gene expression in Ginkgo biloba leaves
摘要
Abstract
By using the open top chambers (OTCs) fumigation method,this paper investigated the changes of foliar injury,level of reactive oxygen species (ROS),activities and gene expression of antioxidant enzymes in Ginkgo biloba leaves under different ozone (ambient ozone≈40,80,160,200 nmol · mol-1) concentrations,in order to study the effects of elevated ozone (O3) concentrations on reactive metabolism.The results showed that the obvious foliar injuries were observed in 160 and 200 nmol mol-1 O3 treatments,while no visible injury was observed in 80 nmol · mol-1 O3 and ambient O3 treatments.After 20 d,a significant increase in O2 generation rate was observed in G.biloba leaves exposed to 160,200 nmol · mol-1 O3,compared with ambient ozone and 80 nmol · mol-1 O3,and there were no significant differences between ambient O3 and 80 nmol · mol-1 treatments.After 40 d,H2O2 content of G.biloba leaves in 160 and 200 nmol · mol-1 O3 was significantly higher than that in 80 nmol · mol-1 and ambient ozone,respectively.The activities of catalase (CAT) in 160 and 200 nmol · mol-1 treatments were also significantly higher than that in 80 nmol · mol-1 and ambient O3 treatments.The ascorbate peroxidase (APX) activity of leaves for each elevated O3 treatment was lower than that of ambient ozone.The level of CAT and APX expression increased progressively after 40 d O3 treatment.The expression intensity of GbD was conspicuously strengthened along with the increase of ozone concentration and fumigation time.Level of reactive oxygen increased,activities of antioxidant enzyme decreased,level of gene expression down-regulated,and foliar visible injury was observed in leaves of G.biloba in elevated ozone stress.关键词
臭氧/银杏/活性氧/基因表达Key words
ozone/Ginkgo biloba/reactive oxygen/gene expression引用本文复制引用
阮亚男,徐胜,郭龙,朱明珠,王聪,李淑媛,王红艳..大气臭氧浓度升高对银杏叶片活性氧代谢及相关基因表达的影响[J].应用生态学报,2017,28(11):3479-3486,8.基金项目
本文由国家自然科学基金项目(31370601,31000225,31670700,41675153)资助 This work was supported by the National Natural Science Foundation of China (31370601,31000225,31670700,41675153). （31370601,31000225,31670700,41675153）
