应用生态学报2017，Vol.28Issue(11)：3521-3526,6.DOI:10.13287/j.1001-9332.201711.007
早实核桃脂肪积累与酶活性动态及其相关性
Dynamics of and relationship between fat accumulation and enzyme activities of precocious walnut
摘要
Abstract
Two precocious walnut varieties,Lvling and Lvzao were used as materials in this study,the kernel fat contents and 3 related enzyme activities in different development periods after flowe ring 50 days were analyzed.The key enzymes affecting walnut fat in different periods were illuminated.The results showed that the kernel fat accumulation trends of the two varieties were basically the same.The kernel began to solidify 50 days after flowering,and the increase in kernel fat content was rapid 60-90 days after flowering,slowed down 90-120 days after flowering,and stopped 120-130 days after flowering.The Logistic model was used to fit the fat accumulation (P＜0.01).Fat content rapid accumulation period was 57.8-85.8 days after flowering for Lvling,and 67.4-92.1 days after flowering for Lvzao.The activities of acetyl-CoA carboxylase (ACCase),6-phosphate dextrose dehydrogenase (G6PDH) and pyruvate kinase (PK) increased from 50 to 100 days after flowering,and then the activities of enzymes began to decrease.The kernel fat content was positively correlated with the activity of ACCase.The kernel fat accumulation rate was positively correlated with PK enzyme activity.The correlation between fat content and enzyme activity was different at different development stages.The 50-100 days after flowering was the exuberant period of walnut kernel fat synthesis,and at this time the fat content could be improved by strengthening the field cultivation management measures.At the early stage of the walnut fat synthesis,G6PDH was the major enzyme to affect fat content,and PK activity influenced the formation of pyruvic acid,so as to indirectly affect the synthesis of fat.ACCase activity affected the final fat content and ACCase played an important regulating role in every period of fat synthesis.It was speculated that ACCase might be a key enzyme to affect the fat synthesis of walnut kernel.关键词
核桃/脂肪/乙酰辅酶A羧化酶/6-磷酸葡萄糖脱氢酶/丙酮酸激酶Key words
walnut/fat/ACCase/G6PDH/Pyruvate Kinase引用本文复制引用
赵爽,刘洋,李寒,陈义兰,李保国,齐国辉,张雪梅..早实核桃脂肪积累与酶活性动态及其相关性[J].应用生态学报,2017,28(11):3521-3526,6.基金项目
本文由“十二五”国家科技支撑计划项目(2013BAD14B03)和河北省科技支撑项目(16236810D)资助 This work was supported by the National Science and Technology Support Program in‘ Twelfth Five-Year’ Period(2013BAD14B03) and the Hebei Science and Technology Support Project (16236810D). （2013BAD14B03）
