应用生态学报2017，Vol.28Issue(11)：3619-3626,8.DOI:10.13287/j.1001-9332.201711.021
干旱条件下钙离子对一氧化氮诱导黄瓜不定根发生的影响
Effects of Ca2+ on nitric oxide-induced adventitious rooting in cucumber under drought stress
摘要
Abstract
Cucumber (Cucumis sativus L.’ Xinchun 4‘) was used to explore the relationship between nitric oxide (NO) and calcium (Ca2+) during adventitious rooting under drought stress.Rooting parameters,endogenous Ca2+ fluorescent intensity and the antioxidant enzymes activity (SOD,CAT and APX) in cucumber explants under drought stress were investigated.The results showed that treatment with 200 μmol · L-1 CaCl2 and 0.05％ PEG significantly improved the number and length of adventitious root in cucumber explants under drought stress,while the application of Ca2+ chelating agent (EGTA) and channel inhibitor (BAPTA/AM) significantly decreased NO-induced number and length of adventitious root under drought stress.Under drought stress,the fluorescence intensity of Ca2+ in hypocotyls treated with NO and CaCl2 was improved,however,the Ca2+ fluorescence intensity in the hypocotyls treated with NO scavenger (cPTIO) was significantly lower than that in the hypocotyls treated with NO.Under drought stress,the activities of antioxidant enzymes in the cucumber explants were significantly promoted by the treatments with NO and CaCl2,however,Ca2+ chelating agent and channel inhibitor significantly decreased the activity of antioxidant enzymes induced by NO.In conclusion,Ca2+ might be involved in the process of NO-adjusted antioxidant enzymes activity during adventitious rooting under drought stress,which alleviated the negative effects of drought on the adventitious rooting and promoted the formation of adventitious roots.关键词
黄瓜/干旱/一氧化氮/钙离子/不定根/抗氧化酶Key words
cucumber/drought/nitric oxide/calcium ion/adventitious root/antioxidant enzymes引用本文复制引用
李春兰,牛丽涓,胡琳莉,廖伟彪,陈悦..干旱条件下钙离子对一氧化氮诱导黄瓜不定根发生的影响[J].应用生态学报,2017,28(11):3619-3626,8.基金项目
本文由国家自然科学基金项目(31160398,31560563)、中国博士后科学基金项目(20100470887,2012T50828)、教育部科学技术研究重点项目(211182)、教育部高校博士点新教师基金项目(201162020005)和甘肃省自然科学基金项目(1308RJZA179,1606RJZA073)资助 This work was supported by the National Natural Science Foundation of China (31160398,31560563),the Post-Doctoral Foundation of China (20100470887,2012T50828),the Key Science and Technology Project of Ministry of Education of China (211182),the Research Fund for the Doctoral Program of Higher Education of Ministry of Education of China (201162020005),the Natural Science Foundation of Gansu Province,China (1308RJZA179,1606RJZA073). （31160398,31560563）
