渔业现代化2017，Vol.44Issue(6)：32-36,42,6.DOI:10.3969/j.issn.1007-9580.2017.06.006
温度对波纹龙虾消化酶活力的影响
Effects of different temperatures on the digestive enzyme activities in the panulirus homarus
摘要
Abstract
To provide an basis for scientific formula of artificial diets,the effects of different temperatures on the digestive enzyme activities in the panulirus homarus were investigated. Using enzymatic analysis method, the four enzymes, including pepsin, trypase, amylase and lipase, were determined in the digestive organs of stomach,intestines,hepatopancreas in the panulirus homarus at temperatures of 20℃,25℃,30℃,35℃,40℃and 45℃. All activities of the four kinds of digestive enzymes presented a trend of first increasing and then decreasing with temperatures ranging from 20℃to 45℃. The highest reaction temperature differed for pepsin reaching the turning point in different digestive organs, with values of 30℃, 35℃, 40℃ corresponded to stomach,intestines and hepatopancreas,respectively. And there were significant differences(P<0. 05)in pepsin among the three tissues,of which stomach presented the maximum level,while hepatopancreas had the lowest one. The temperature for the presentation of the maximal activity of trypsase in different digestive organs were same at 40℃,and level of trypsase in stomach was significantly lower P<0. 05 ) than those in intestines and hepatopancreas,with the interval was up to 40 U/mg. The amylase activity exhibited maximal in intestine, which differed significantly( P<0. 05) from those in other two organs,but the highest reaction temperature for activity of amylase in all three digestive organs were 25℃. The activities of lipase in all digestive organs had the same optimal reaction temperature of 30℃, when the activity of lipase was highest in hepatopancreas, whereas stomach presented a lower lipase activity(P<0. 05).关键词
波纹龙虾/温度/消化酶/活力Key words
panulirus homarus/temperature/digestive enzyme/activity分类
农业科技引用本文复制引用
黄东科,梁华芳,温崇庆,廖永岩..温度对波纹龙虾消化酶活力的影响[J].渔业现代化,2017,44(6):32-36,42,6.基金项目
广西北部湾海洋生物多样性养护重点实验室(钦州学院)开放课题(2015KB03) （钦州学院）
广东省科技厅农业攻关项目(2013B020308009) （2013B020308009）
