针刺研究2017，Vol.42Issue(6)：471-476,6.DOI:10.13702/j.1000-0607.2017.06.001
麦粒灸预处理对大鼠心肌缺血再灌注损伤不同时期保护效应及Beclin1表达的影响
Effect of Moxibustion Preconditioning with Seed-sized Moxa Cones on Myocardial Infarction Size and Beclin 1 Expression in Myocardial Ischemia-reperfusion Injury Rats
摘要
Abstract
Objective To observe the effect of moxibustion (Moxi) preconditioning with seed-sized moxa cones on myocardial ischemia-reperfusion injury (MI/RI) at different stages,and to analyze the correlation between this effect and the expression of autophagy related protein Beclin 1.Methods This study contains two parts:1) changes of myocardial pathological injury and percentages of myocardial infarcted area at different time-points after modeling and Moxi intervention,and 2) effect of Moxi on contents of serum cardiac troponin T(cTnT) and expression of myocardial Bcl-2,Bax and Beclin 1 proteins.In the first part,42 SD rats were randomly divided into model group,1 day (d) Moxi group,2 d Moxi group,3 d Moxi group,4 d Moxi group,5 d Moxi group and 7 d Moxi group.The model of MI/RI was established by ligating the left anterior descending coronary artery (LAD) for 30 min and reperfusion for 240 min.The electrocardiogram (ECG) of standard limb lead Ⅱ was monitored and the heart was taken 4 h after reperfusion for examining myocardial infarcted size with triphenyl tetrazolium chloride (TTC) staining.In the second part,48 SD rats were randomized into sham-operation,model,moxibustion and autophagy inhibitor (3-MA) groups,with 12 rats in each group.The serum cTnT level was assayed and histopathological changes of the myocardial tissue below the ligation site were examined with HE staining,and the expression levels of Bcl-2,Bax and Beclin 1 proteins in the myocardial tissue below the LAD-ligated site were detected using Western blot.Results Compared with the model group,the percentages of myocardial infarcted area were significantly decreased in the 4 d,5 d and 7 d Moxi groups (P＜0.05),but without significant differences among the 3 Moxi groups (P＞0.05).The state of MI-induced breakage and disordered arrangement of myocardial fibers with interstitial edema and inflammatory cell infiltration at the MI stage in the Moxi group and at the reperfusion stage in the autophagy inhibitor group was relatively lighter.The levels of serum cTnT content and Bax/Bcl-2 and Beclin 1 protein expression at the MI and reperfusion stages were significantly higher in the model group than in the sham-operation group (P＜0.01),and considerably lower in the Moxi and autophagy groups than in the model group (P＜0.01).The serum cTnT content,ratio of Bax/Bcl-2 expression and Beclin 1 expression levels at the MI and reperfusion stages were significantly lower in the autophagy inhibitor group than in the Moxi group (P＜0.05).Conclusion Moxibustion with seed-sized moxa cones at "Neiguan" (PC 6) can effectively alleviate myocardial ischemia in MI/RI rats,which is probably related to its effect in down-regulating Bax/Bcl-2 and Beclin 1 expression and in inhibiting autophagy.关键词
心肌缺血再灌注/麦粒灸/自噬/自噬相关蛋白Beclin 1Key words
Myocardial ischemia reperfusion/Moxibustion with seed-size moxa cone/Autophagy/Autophagy associated protein Beclin 1分类
医药卫生引用本文复制引用
白桦,卢圣锋,陈婉莹,仲泽昊,顾一煌..麦粒灸预处理对大鼠心肌缺血再灌注损伤不同时期保护效应及Beclin1表达的影响[J].针刺研究,2017,42(6):471-476,6.基金项目
国家自然科学基金项目(No.81674063) （No.81674063）
江苏省普通高校科研创新计划项目(No.KYLX 16-1152) （No.KYLX 16-1152）
