针刺研究2017，Vol.42Issue(6)：477-481,5.DOI:10.13702/j.1000-0607.2017.06.002
电针与黄芪甲苷结合对大鼠心肌纤维化的影响
Effect of Combined Intervention of Electroacupuncture and Astragaloside Ⅳ on Myocardial Hypertrophy and TGF-β 1/Smad Signaling in Rats with Myocardial Fibrosis
摘要
Abstract
Objective To observe the effect of combined intervention of electroacupuncture (EA) and astragaloside Ⅳ (ASIV) on cardiac hypertrophy and transforming growth factor β 1 (TGF-β 1)/Smad signaling in isoproterenol (ISO) induced cardiac hypertrophy rats,so as to investigate its underlying mechanisms in improving myocardial fibrosis.Methods A total of 50 SD rats were randomly divided into 5 groups:normal control,model (ISO),Propranolol (PRO),ASIV and EA+ ASIV groups (n =10 in each group).The myocardial fibrosis model was established by intraperitoneal injection (i.p.) of ISO (10 mg · kg-1 · d-1),once daily for 30 days.Rats of the control group were given normal saline (i.p.),those of the PRO group given with PRO (40 mg · kg-1 · d-1,gavage),and those of the ASIV and EA+ASIV groups were treated by gavage of ASIV (40 mg · kg-1 · d-1),once daily for 30 days.EA (20 Hz,6 V) was applied to bilateral "Neiguan" (PC 6) for 10 min,once every day for 30 d.The heart mass index (HMI,whole heart weight/body weight) and left ventricular (LV) mass index (LVMI,weight of the LV/body weight) were calculated to assess the state of cardiac hypertrophy.The enzyme linked immunosorbent assay (ELISA) was used to determine the levels of procollagen Ⅰ carboxy-terminal propeptide (PICP,a marker of extracellular matrix remodeling) and carboxyterminal telopeptide of type Ⅰ collagen (ICTP,a metabolite of type Ⅰ collagen) in serum,and Western blot was used to test protein contents of TGF-β 1,Smad 2 / 3,Smad 4,Smad 7 in the left ventricle tissue of the heart.Results After modeling,the HMI and LVMI,serum PICP and ICTP contents and the expression levels of myocardial TGF-β 1,Smad 2/3 and Smad 4 proteins were significantly increased in the model (ISO) group (P＜0.05),suggesting a deposition of collagen and cardiac hypertrophy,and were considerably decreased in PRO,ASIV and EA+ ASIV groups after the intervention (P＜0.05).The expression level of myocardial Smad 7 protein was significantly lower in the model group than in the normal control group (P＜0.05),and significantly up-regulated in PRO,ASIV and EA+ ASIV groups (P＜0.05).Sirius Red staining of the left ventricular myocardium showed a dense deposition of collagen and a severer myocardial fibrosis in the model group,and a relatively lighter fibrosis in the PRO,ASIV and EA+ ASIV groups.The therapeutic effects of EA+ ASIV were comparable to those of PRO,and were significantly superior to those of ASIV in down-regulating HMI,serum ICTP,and myocardial Smad 2/3 and Smad 4 expression and up-regulating Smad 7 protein (P＜0.05).There were no significant differences among the PRO,ASIV and EA+ ASIV groups in LVMI,PICP and TGF-β 1 levels,and between the PRO and EA+ ASIV groups in HMI,ICTP,Smad 2/3,Smad 4 and Smad 7 levels (P＞ 0.05).Conclusion EA stimulation of PC 6 combined with ASIV can relieve cardiac hypertrophy and myocardial fibrosis in rats,which may be associated with its effects in regulating myocardial TGF-β 1/Smad signaling pathway.关键词
心肌纤维化/电针/黄芪甲苷/普萘洛尔/心肌TGF-β1/Smad信号通路Key words
Myocardial fibrosis/Electroacupuncture/Astragaloside Ⅳ/Propranolol/Myocardial TGF-β1/Smad signaling pathway分类
医药卫生引用本文复制引用
李佳莘,朱晓雨,鲁美丽,高俊虹,王洪新,喻晓春..电针与黄芪甲苷结合对大鼠心肌纤维化的影响[J].针刺研究,2017,42(6):477-481,5.基金项目
中国中医科学院针灸研究所基本科研业务费自主选题项目(No.ZZKF 08005) （No.ZZKF 08005）
