针刺研究2017，Vol.42Issue(6)：489-495,7.DOI:10.13702/j.1000-0607.2017.06.004
电针干预对大鼠腓肠肌适应性肥大及肌卫星细胞增殖分化的影响
Effects of Electroacupuncture on Adaptive Hypertrophy of Gastrocnemius and Proliferation and Differentiation of Muscle Satellite Cells in Rats
摘要
Abstract
Objective To explore the effects and mechanism of different duration electroacupuncture (EA) on adaptive hypertrophy of gastrocnemius and the expressions of proliferation cell nuclear antigen (PCNA),paired box transcription factor 7 (Pax 7),myogenic differentiation antigen (MyoD 1),myogenin (MyoG),myosin heavy chain-Ⅰ (Myh 7),transforming growth factor-β 1 (TGF-β 1),Smad 3 in rats.Methods Twenty-four SD adult male rats were randomly divided into normal,two-week EA (A 2),four-week EA (A 4) and eight-week EA (A 8) groups,6 rats in each group.Except the normal group,EA was applied at right "Zusanli" (ST 36) and "Huantiao" (GB 30) for 10 min in the other three groups,separately for 2 weeks,4 weeks and 8 weeks,once a day,6 times a week.The ratio of gastrocnemius wet weight was calculated after EA.The cross-sectional area and diameter of muscle fiber were measured after HE staining.The expression of PCNA protein was detected by irnmunofluorescence.The relative expressions of Pax 7,MyoD 1,MyoG,Myh 7,TGF-β 1 and Smad 3 mRNA were tested with real-time PCR.Results The expressions of PCNA protein were obviously higher in the A 2 and A 4 groups than that in the normal group.The expression of genes in the EA intervention groups showed a trend of increasing first and then decreasing except Smad 3 mRNA.The relative expressions of Pax 7 and TGF-β 1 mRNAs reached their peaks in the A 2 group compared with those in the normal group (P＜ 0.01).The ratio of gastrocnemius wet weight,cross-sectional area and diameter of muscle fiber,and the relative expressions of MyoD 1,MyoG,Myh 7 mRNAs reached their peaks in the A 4 group (P＜0.01),while the relative expression of Smad 3 mRNA showed a trend of decreasing first and then increasing and reached its bottom at the 4th week (P＜0.01).Conclusion EA at "Zusanli" (ST 36) and "Huantiao" (GB 30) may induce adaptive hypertrophy of gastrocnemius in rats by up-regulating the expressions of Pax 7,PCNA,TGF-β 1,MyoD 1,MyoG,Myh 7 and down-regulating the expression of Smad 3,promoting the proliferation of skeletal satellite cells,myogenic differentiation,myotubes terminal differentiation,increasing the wet weight,fiber crosssectional area and diameter of gastrocnemius.关键词
电针/肌卫星细胞/骨骼肌肥大/配对盒转录因子7/成肌分化抗原Key words
Electroacupuncture/Muscle satellite cells/Hypertrophy of skeletal muscle/Paired box transcription factor 7/Myogenic differentiation antigen分类
医药卫生引用本文复制引用
赵丹丹,唐成林,黄思琴,罗翱,张安宁,郭全虎,曹净,高睿琦..电针干预对大鼠腓肠肌适应性肥大及肌卫星细胞增殖分化的影响[J].针刺研究,2017,42(6):489-495,7.基金项目
国家自然科学基金(No.81273870) （No.81273870）
