针刺研究2017，Vol.42Issue(6)：507-509,3.DOI:10.13702/j.1000-0607.2017.06.007
针刺跷脉对失眠大鼠视交叉上核内生物钟基因Period1及Period2的影响
Effect of Acupuncture at Points in Heel Vessel for Circadian Clock Genes of Period 1 and Period 2 mRNAs in the Suprachiasmatic Nucleus in Insomnia Rats
摘要
Abstract
Objective To investigate the influence and mechanism of acupuncture at the points in Heel Vessel for the circadian clock genes of Period (Per) 1 and Per 2 mRNAs in the suprachiasmatic nucleus (SCN) in insomnia rats.Methods Thirty male SD rats were randomly divided into blank,model,acupuncture groups,10 rats in each group.Insomnia model was established by intraperitoneal injection of PCPA (suspension,1 mL/100 g).Acupuncture at "Shenmai" (BL 62) and "Zhaohai" (KI 6) was used in the acupuncture group for continuous 7 days,15 min/day and once daily.The circadian rhythm was observed;the expressions of Per 1 and Per 2 mRNAs in SCN were examined with real time-PCR.Results The activity in the model group in rest period everyday increased compared with that in the blank group,and the expressions of Per 1 and Per 2 mRNAs in SCN decreased (P＜0.05).Compared with the model group,the activity in the acupuncture group in rest period decreased and the expressions of Per 1 and Per 2 mRNAs in the SCN increased (P＜0.05).Conclusion Acupuncture at BL 62 and K I 6 can increase the expressions of Per 1 and Per 2 mRNAs in the SCN,so as to decrease the activity in rest period,and improve the quality of sleep in insomnia rats.关键词
失眠/跷脉/针刺/睡眠-觉醒周期Key words
Insomnia/Heel Vessel/Acupuncture/Sleep-wake cycle分类
医药卫生引用本文复制引用
郭保君,余思奕,申治富,胡幼平..针刺跷脉对失眠大鼠视交叉上核内生物钟基因Period1及Period2的影响[J].针刺研究,2017,42(6):507-509,3.基金项目
国家自然科学基金面上项目(No.81373560) （No.81373560）
