针刺研究2017，Vol.42Issue(6)：510-513,556,5.DOI:10.13702/j.1000-0607.2017.06.008
电针对偏头痛大鼠中脑导水管周围灰质5-羟色胺7受体和血浆降钙素基因相关肽表达的影响
Effect of Electroacupuncture on Expression of 5-HT7 Receptor in Periaqueductal Gray and Plasma Calcitonin Gene-related Peptide in Migraine Rats
摘要
Abstract
Objective To explore the analgesic effect of electroacupuncture(EA)by modulating 5-hydroxytryptamine 7 (5-HT7) receptor in periaqueductal gray (PAG) and plasma calcitonin gene-related peptide (CGRP).Methods Forty-two male Sprague Dawley (SD) rats were randomly divided into control,model and EA groups,14 rat in each one.The neurogenic migraine model was established by repeated electrical stimulation on sagittal sinus duramater.Intracranial electrodes were used in the control group without stimuli.The rats in the EA group received EA (0.5-1 mA,2 Hz/15 Hz) at "Fengchi" (GB 20) for 10 min after dural electrical stimulation,once a day for 6 days.The expression of 5-HT7 receptor in the PAG was assessed by immunofluorescence and Western blot,respectively;plasma CGRP was measured by radioimmunoassay.Results Compared with the control group,the positive neuron number and protein expression of 5-HT7 receptor in PAG and plasma CGRP increased after model establishment (all P＜0.001).The above mentioned indexes were reversed in the EA group compared with those in the model group (the positive neuronnumber and protein expression of 5-HT7 receptor,P＜0.01;plasmaCGRP,P＜0.05).Conclusion EAatGB20 can down-regulate the expression of 5-HT7 receptor in the PAG and reduce the content of plasma CGRP in the rats of migraine.关键词
偏头痛/电针/中脑导水管周围灰质/5-羟色胺7受体Key words
Migraine/Electroacupuncture/Periaqueductal gray (PAG)/5-HT7 receptor分类
医药卫生引用本文复制引用
裴培,陈怀珍,王艳昕,杨文明,刘璐,王麟鹏..电针对偏头痛大鼠中脑导水管周围灰质5-羟色胺7受体和血浆降钙素基因相关肽表达的影响[J].针刺研究,2017,42(6):510-513,556,5.基金项目
国家重点基础研究发展计划“973”中医专项(No.2014 CB 543203) （No.2014 CB 543203）
国家自然科学基金资助项目(No.81603683) （No.81603683）
