针刺研究2017，Vol.42Issue(6)：514-517,4.DOI:10.13702/j.1000-0607.2017.06.009
针刀干预对颈椎病兔颈后伸肌B淋巴细胞瘤-2及其相关的x基因、天冬氨酸特异性半胱氨酸蛋白酶-3mRNA表达的影响
Effects of Acupotomy Therapy on mRNA Expressions of Bcl-2, Bax, Caspase-3 in Posterior Cervical Extensor Muscles in Cervical Spondylosis Rabbits
摘要
Abstract
Objective To observe the effect of acupotomy therapy on mRNA expressions of Bcl-2,Bax,Caspase-3 in posterior cervical extensor muscles in cervical spondylosis rabbits,and explore its mechanisms for apoptosis of cervical muscles.Methods New Zealand rabbits were randomly divided into normal,model,electroacupuncture (EA) and acupotomy groups,6 in each group.The cervical spondylosis model was established by forced head-bowing for a long term.After model establishment,acupotomy was used at the trapezius muscle starting point and attachment site of sternocleidomastoid muscle,etc.,once a week,total 3 times.EA was used at "Tianzhu" (BL 10),"Jingbailao" (EX-HN 15) and "Dazhu" (BL 11) for 3 weeks,20 min a time,3 times a week.Bcl-2,Bax,Caspase-3 mRNA expressions in posterior cervical extensor muscles were detected by real-time PCR.Results There was no significant difference in the expression of Bcl-2 mRNA among all the groups (P＞0.05).Compared with the normal group,Bax mRNA increased in the model group (P＜0.01),and that in the acupotomy group was lower than that in the model group (P＜0.01).The ratio of Bcl-2/Bax mRNA in the model group decreased significantly compared with that in the normal group (P＜0.01);in comparison with the model group,the ratio in the acupotomy group increased (P＜0.01);and that in the acupotomy group was higher than the ratio in the EA group (P＜0.05).The Caspase-3 mRNA expression in the model group increased compared with that in the normal group (P＜0.05),and its expression in the acupotomy group decreased compared with those in the model and EA groups (P＜0.05).Conclusion Acupotomy therapy can regulate the mRNA expressions of Bax and Caspase-3,and retard apoptosis in posterior cervical extensor muscles,therefore the strained muscles are relieved,which may be one of its mechanisms for improving cervical spondylosis.关键词
针刀/颈椎病/细胞凋亡/B淋巴细胞瘤-2基因/13淋巴细胞瘤-2相关的x基因/天冬氨酸特异性半胱氨酸蛋白酶-3基因Key words
Acupotomy/Cervical spondylosis/Apoptosis/Bcl-2 mRNA/Bax mRNA/Caspase-3 mRNA分类
医药卫生引用本文复制引用
刘福水,周凡媛,张义,郭长青..针刀干预对颈椎病兔颈后伸肌B淋巴细胞瘤-2及其相关的x基因、天冬氨酸特异性半胱氨酸蛋白酶-3mRNA表达的影响[J].针刺研究,2017,42(6):514-517,4.基金项目
国家自然科学基金(No.81560792) （No.81560792）
江西省教育厅科技计划项目(重点项目,No.GJJ 150834) （重点项目,No.GJJ 150834）
江西省卫生计生委中医药科研项目(重点课题,No.2016 Z 03) （重点课题,No.2016 Z 03）
