中国动脉硬化杂志2017，Vol.25Issue(11)：1081-1087,7.
血管生成素1通过LXRα途径调节THP-1源性泡沫细胞ABCA1、ABCG1表达及胆固醇流出
Angiopoietin-1 mediates ABCA1, ABCG1 expression and cholesterol efflux through LXRα pathway in THP-1 derived macrophages
摘要
Abstract
Aim To investigate whether angiopoietin-1 (Ang-1) regulates ATP-binding cassette transporter A1 (ABCA1),ABCG1 expression and cholesterol efflux through liver X receptor α (LXRαt) in THP-1 derived macrophages.Methods THP-1 cells were incubated with 160 nmol/L phorbol ester to transform into macrophages.Subsequently,THP-1 derived macrophages were exposed to 50 mg/L oxidized low density lipoprotein (ox-LDL) and cultured with typical approaches.The experimental groups were divided into control group and Ang-1 group.Liquid scintillation counter was used to determine the efficiency of cholesterol efflux.Lipids contents were tested with high performance liquid chromatogram (HPLC).Real-time PCR and Western blot were used to quantify the expression of ABCA1,ABCG1.THP-1 derived macrophages were pretreated with LXRα angonists T0901317 or inhibitors GGPP,respectively.Results Ang-1 obviously reduced cholesterol efflux,and downregulated ABCA1,ABCG1 expression.LXRα angonists T0901317 interfered Ang-1-inhibited ABCA1,ABCG1 expression and cholesterol efflux.Conclusion Ang-1 decreased THP-1 derived macrophages cholesterol efflux in a LXRα dependent manner and downregulated ABCA1,ABCG1 expression.关键词
血管生成素1/三磷酸腺苷结合盒转运体A1/三磷酸腺苷结合盒转运体G1/胆固醇流出/肝X受体αKey words
Angiopoietin-1/ATP-binding cassette A1/ATP-binding cassette G1/Cholesterol efflux/Liver X receptor α分类
医药卫生引用本文复制引用
欧翔,陈凌燕,周志姣,夏晓丹,龚朵,赵真旺,王斯琦,张强,唐朝克..血管生成素1通过LXRα途径调节THP-1源性泡沫细胞ABCA1、ABCG1表达及胆固醇流出[J].中国动脉硬化杂志,2017,25(11):1081-1087,7.基金项目
国家自然科学基金项目(81570408) （81570408）
湖南省自然科学基金(2017JJ2286) （2017JJ2286）
长沙市科技计划项目(KQ1602028) （KQ1602028）
