中国动脉硬化杂志2017，Vol.25Issue(11)：1088-1092,5.
护骨素信号介导Ghrelin调控A7r5主动脉平滑肌细胞钙化
Study of Ghrelin regulating calcification in A7r5 aortic smooth muscle cells mediated by osteoprotegerin signaling
摘要
Abstract
Aim To observe the correlative mechanism between ghrelin and osteoprotegerin (OPG)/receptor activator of nuclear factor kappa B ligand (RANKL) in A7r5 calcification.Methods Changes of ghrelin in A7r5 calcification under high-lipid,glucose-coexisting conditions were observed.And then,effects of OPG and RANKL regulated by exogenous ghrelin on A7r5 calcification under high-lipid,glucose,β-glycerophosphate-coexisting conditions were investigated.Finally,the interaction between OPG/RANKL and ghrelin in A7r5 calcification under high-lipid,glucose,β-glycerophosphate-coexisting conditions was explored.Von Kossa staining,alizarin red staining,extracellular calcium deposition assay,immunohistochemical staining,Western blot analysis were performed after incubation of 14 days.Results Von Kossa staining,alizarin red staining and extracellular calcium deposition test showed that calcium deposition of A7r5 cells significantly increased by 5.21 times under diabetic metabolic disorder simulated by high-lipid,glucose,β-glycerophosphate-coexisting conditions.Ghrelin immunohistochemical staining and IPP6 quantitative analysis indicated that the expression of ghrelin was dramatically reduced with the formation and development of calcification.Exogenous ghrelin could increase the relative expression of OPG by 6.25 times.Compared with the high glucose,high lipid and calcified group,the calcium deposition in anti-OPG group and RANKL group were raised by 1.33 times (P＜0.05) and 1.59 times (P＜0.05),respectively.Compared with anti-OPG group,the calcium deposition in anti-OPG plus ghrelin group was decreased by 41.9％ (P＜0.05);Compared with RANKL group,the calcium deposition in RANKL plus ghrelin group was decreased by 57.8％ (P＜0.05).Conclusion Ghrelin regulated A7r5 cells calcification by OPG/RANKL signal.关键词
胃促生长素/护骨素/细胞核因子κB受体活化因子配体/血管钙化Key words
Ghrelin/Osteoprotegerin/Nuclear factor κB receptor activator ligand/Vascular calcification分类
医药卫生引用本文复制引用
戴俏武,王中群,严金川,邵晨,孙振,包正阳,景乐乐,李丽华..护骨素信号介导Ghrelin调控A7r5主动脉平滑肌细胞钙化[J].中国动脉硬化杂志,2017,25(11):1088-1092,5.基金项目
国家自然科学基金(81370408,81770450) （81370408,81770450）
江苏省自然科学基金(BK20131246) （BK20131246）
江苏省卫计委项目(Q201308,QNRC2016836) （Q201308,QNRC2016836）
江苏省研究生科研创新计划(KYCX17_1801) （KYCX17_1801）
镇江市社会发展项目(SH2015038,SH2015023) （SH2015038,SH2015023）
江苏大学临床专项(JDLCZX001) （JDLCZX001）
