局解手术学杂志2017，Vol.26Issue(10)：710-714,5.DOI:10.11659/jjssx.06E017063
L-左旋肌肽对人肝癌细胞HepG2增殖及凋亡作用的实验研究
Study in L-carnosine on the proliferation and apoptosis of human hepatocellular carcinoma HepG2 cells
摘要
Abstract
Objective To study the L-carnosine induced apoptosis of human hepatocellular carcinoma HepG2 cells and its mechanism.Methods Human hepatocellular carcinoma HepG2 cells were cultured in vitro,then the cells in logarithmic phase were divided into 5 mM,20 mM,50 mM L-carnosine group and control group,treated with 5 mM,20 mM,50 mM L-carnosine and saline respectively.The proliferation of HepG2 cells was measured by CCK-8 assay after 24 hours and 48 hours treatment.The cell apoptosis and cell cycle were detected by flow cytometry after 48 hours treatment.The protein expression of Caspase-8,PI3K and Akt were detected by Western blot in each group after 48 hours treatment.Results CCK-8 and flow cytometry assay showed that both 20 mM and 50 mM L-carnosine treated group significantly inhibited the proliferation of HepG2 cells after 24 hours and 48 hours,and the inhibition were in a time and dose-dependent manner.Western blot revealed that the expressions of PI3K and Akt were down-regulated with the increase of L-carnosine concentration,while the expression of Caspase-8 was increased.Conclusion L-carnosine exhibits an inhibitory effect of the proliferation and promote apoptosis of human liver cancer cell line HepG2.The mechanism may be associated with the inhibition of the PI3K/Akt signaling pathway and activation of Caspase-8 signaling pathway.关键词
L-左旋肌肽/肝细胞癌/凋亡/增殖/PI3K/AktKey words
L-carnosine/hepatocellular carcinoma/apoptosis/proliferation/PI3K/Akt分类
医药卫生引用本文复制引用
刘强,彭玉萍,戴社教,马小斌..L-左旋肌肽对人肝癌细胞HepG2增殖及凋亡作用的实验研究[J].局解手术学杂志,2017,26(10):710-714,5.基金项目
陕西省社会发展科技攻关项目(2013k12-03-20) （2013k12-03-20）
