局解手术学杂志2017，Vol.26Issue(10)：714-718,5.DOI:10.11659/jjssx.05E017005
维生素D调控龟板提取物促骨髓间充质干细胞增殖分化的研究
Vit D promotes proliferation and differentiation of mesenchymal stem cells through regulates extracts of plastrum testudinis
摘要
Abstract
Objective To explore the effect and the mechanism of vitamin D(Vit D) promotes proliferation and differentiation of mesenchymal stem cells (MSCs) through regulates extracts of plastrum testudinis (PTE).Methods Established the PGL3-Id1 promoter and transfered rat MSCs.PTE combined with 10-6,10-7,10-8mol/L Vit D respectively were acted on the transfected MSCs for 36 hours.The level of Id1 promoter were detected by luciferase activity measurement.1,3,30,100 pg/mL PTE combined with Vit D of 10-7 mol/L were acted on MSCs for 36 hours,3 days and 7 days,and the VDR expression were detected by RT-PCR test.Results PTE promoted the expression of Id1 in MSCs,the expression of Id1 was inhibited when PTE combined with Vit D (P ＜ 0.01),and it was significantly different among different dosis of Vit D(P ＜0.01).The expression of VDR was inhibited in different degree when PTE combined with Vit D for 36 hours,3 days and 7 days.PTE combed with large dose of Vit D for 36 hours had significant effect of inhibition,and the difference was statistically significant (P ＜ 0.05).The inhibiting effect was more obvious when PTE combined with large dose of Vit D for 3 days and 7 days.When different doses of PTE combined with Vit D for a same duration,the difference of VDR expression was statistically significant (P ＜ 0.05).Meanwhile,when same doses of PTE combined with Vit D for different durations,the difference of VDR expression at 7 days and 36 hours was statistically significant (P ＜ 0.05).Conclusion The proliferation of MSCs which promoted by PTE was inhibited by Vit D,and the nuclear receptor VDR may be one of the targets of drug action for PTE regulating proliferation and differentiation of MSCs.关键词
龟板提取物/骨髓间充质干细胞/维生素D/VDRKey words
extracts of plastrum testudinis/bone marrow mesenchymal cell/Vitamin D/VDR分类
医药卫生引用本文复制引用
陈金锋,张海玲,肖春苟,叶茂盛,郑二来,陈东风..维生素D调控龟板提取物促骨髓间充质干细胞增殖分化的研究[J].局解手术学杂志,2017,26(10):714-718,5.基金项目
广东省中医药管理局课题(20141300) （20141300）
肇庆市科技创新计划项目(2013E2810) （2013E2810）
