局解手术学杂志2017，Vol.26Issue(10)：719-723,5.DOI:10.11659/jjssx.05E017009
AICAR通过提高MSC移植存活率促进糖尿病溃疡创面愈合的研究
AICAR promotes the wound healing in diabetic ulcers by improving the survival rate of MSC in biomaterials transplantation
柏力 1李刚 2李立 2潘险峰1
作者信息
- 1. 解放军昆明总医院急救部,云南昆明650032
- 2. 第三军医大学人体解剖学教研室,重庆400038
- 折叠
摘要
Abstract
Objective To investigate the mechanism of AICAR in improving the survival of mesenchymal stem cells (MSCs) in biomaterials and the therapeutic effect on diabetic ulcer.Methods Totally 40 male C57BL/6J mice were equally divided into 4 groups:control group,MSCs group,AICAR group and AICAR-MSCs group.The effects of AICAR on the apoptosis and migration of MSCs were observed by flow cytometry and transwell migration assay.The expression of VEGF in conditioned medium was detected.Prepared diabetic foot ulcer model and AICAR-stimulated MSCs-covered wounds.The wound neovascularization was observe by CD31 staining 2 weeks later.Results Compared with the control group and MSCs group,the results of the cell experiments showed that the AICAR-MSCs group had lower MSCs apoptosis rate,better MSCs migration ability and higher VEGF secretion.Animal experiments showed that AICAR-stimulated MSCs covered diabetic foot ulcer wounds had good angiogenesis and rapid ulcer healing processes.Conclusion AICAR promotes the survival and paracrine effect of MSCs by inhibiting the high glucose effect on MSCs apoptosis,so as to promote the angiogenesis and accelerate the healing of ulcer.关键词
阿卡地新/间充质干细胞/血管新生/糖尿病足溃疡创面Key words
AICAR/mesenchymal stem cells/angiogenesis/diabetic foot ulcer分类
医药卫生引用本文复制引用
柏力,李刚,李立,潘险峰..AICAR通过提高MSC移植存活率促进糖尿病溃疡创面愈合的研究[J].局解手术学杂志,2017,26(10):719-723,5.
