中国康复理论与实践2017，Vol.23Issue(12)：1365-1371,7.DOI:10.3969/j.issn.1006-9771.2017.12.001
针康法对脑缺血大鼠缺血半暗区细胞凋亡及X连锁凋亡抑制蛋白和cleaved-caspase-9蛋白表达的影响
Effects of Acupuncture-rehabilitation Therapy on Apoptosis and Expression of X-linked Inhibitor of Apoptosis Protein and Cleaved-caspase-9 in Ischemic Penumbra of Rats with Cerebral Ischemia
摘要
Abstract
Objective To explore the effect of acupuncture-rehabilitation therapy on apoptosis and the expression of X-linked inhibitor of apoptosis protein (XIAP) and cleaved-caspase-9 in ischemic penumbra of rats with cerebral ischemia. Methods A total of 180 male Sprague-Dawley rats were randomly divided into sham operation group,model group,acupuncture group,rehabilitation group and acupunc-ture-rehabilitation therapy group.Each group was divided into three days,seven days and 14 days subgroups(n=12).The model was estab-lished with the modified Longa suture method. The model group and the sham operation group received no treatment. The acupuncture group received cluster needling of scalp acupuncture,the rehabilitation group received treadmill training,and the acupuncture-rehabilitation therapy group received both acupuncture and treadmill training.On the third,seventh and 14th days after modeling,the neurological func-tion was assessed with modified Neurological Severity Score(mNSS).The apoptosis was observed by TUNEL staining and the expression of XIAP and cleaved-caspase-9 protein was determined by Western blotting in cerebral ischemic penumbra,respectively.Results Compared with the model group,the mNSS scores decreased,the rate of apoptosis decreased,the expression of XIAP protein increased,and the expres-sion of cleaved-caspase-9 decreased in each treatment group at each time point(P<0.05).Compared with the other two treatment groups,the rate of apoptosis further decreased at each time points(P<0.05),the expression of XIAP protein further increased at each time points(P<0.05),the mNSS score further decreased(P<0.05)and the expression of cleaved-caspase-9 protein further decreased(P<0.05)seven days and 14 days after modeling in the acupuncture-rehabilitation therapy group.Conclusion Acupuncture and rehabilitation therapy could re-duce the neurological deficit in rats with cerebral ischemia,which was superior to the simple acupuncture treatment and rehabilitation train-ing.The mechanism may be related to th reduction of apoptosis by promoting XIAP protein expression and inhibiting caspase-9 protein acti-vation.关键词
脑缺血/神经功能/针康法/凋亡/X连锁凋亡抑制蛋白/cleaved-caspase-9/大鼠Key words
cerebral ischemia/neurological function/acupuncture-rehabilitation therapy/apoptosis/X-linked apoptosis inhibitory pro-tein/cleaved-caspase-9/rats分类
医药卫生引用本文复制引用
唐强,田源,叶涛,朱路文,阮野,赵彬,陈慧杰,王雪..针康法对脑缺血大鼠缺血半暗区细胞凋亡及X连锁凋亡抑制蛋白和cleaved-caspase-9蛋白表达的影响[J].中国康复理论与实践,2017,23(12):1365-1371,7.基金项目
1.国家自然科学基金项目(No.81473762 （）
No.81273818) （）
2.黑龙江中医药大学领军人才计划项目(No.2012RCL02) （No.2012RCL02）
3.黑龙江省高校科技创新团队计划项目(No.2013TD007). （No.2013TD007）
