中国康复理论与实践2017，Vol.23Issue(12)：1384-1389,6.DOI:10.3969/j.issn.1006-9771.2017.12.004
丰富环境对血管性痴呆大鼠侧脑室室管膜下区神经再生的影响
Effect of Enriched Environment on Neuroregeneration in Subventricle Zone of Rats with Vascular Dementia
摘要
Abstract
Objective To explore the effect of enriched environment on neuroregeneration in subventricle zone(SVZ)of rats with vascu-lar dementia.Methods All rats were divided into sham group(n=5),vascular dementia group(VD group,n=12)and enriched environment group(EE group,n=12).Then,2-VO method was applied to make the vascular dementia model.The sham group and VD group received conventional breeding environment for 30 days,while EE group was subjected to enriched environment for 30 days.Immunohistochemistry was applied to detect the BrdU and Doublecortin(DCX)expression in SVZ,and DCX/Ki67,DCX/p-cAMP-response element binding pro-tein(CREB)expression in SVZ was assessed by immunofluorescence double-labeling technique.Results Compared with the sham group, the number of DCX+cells(t=2.989,P=0.026)and BrdU+cells(t=3.069,P=0.005)increased in VD group,and increased more in EE group (t=3.067, P=0.027; t=2.907, P=0.011). Besides, the number of the DCX/Ki67 (t=2.994, P=0.040) and DCX/p-CREB (t=4.707, P=0.009) cells was significantly higher in EE group than in VD group.Conclusion Enriched environment could up-regulate the neuroregeneration ca-pacity in SVZ of rats with vascular dementia through CREB signal pathway.关键词
血管性痴呆/丰富环境/室管膜下区/神经再生/大鼠Key words
vascular dementia/enriched environment/subventricle zone/neuroregeneration/rats分类
医药卫生引用本文复制引用
贺旭,刘英飞,罗明英,成绍武,葛金文..丰富环境对血管性痴呆大鼠侧脑室室管膜下区神经再生的影响[J].中国康复理论与实践,2017,23(12):1384-1389,6.基金项目
1.国家自然科学基金项目(No.81500377) （No.81500377）
2.湖南省教育厅优秀青年课题(No.16B269) （No.16B269）
3.益阳医学高等专科学校引进高层次人才科研启动费项目(No.2016-001). （No.2016-001）
