中国临床药理学与治疗学2017，Vol.22Issue(9)：972-977,6.
Ero1α在缺氧复氧致H9C2细胞内质网应激时的表达变化及意义
Change and significance of Ero1α expression on endoplasmic reticulum stress induced by hypoxia/reoxygenation in rat cardiomycytes
摘要
Abstract
AIM:To explore the relationship between Ero1α expression and ERS and apoptosis in rat hypoxia/reoxygenation cardiomyocyte model.METHODS:The H9C2 cardiomyocytes were randomly divided into groups:control group,hypoxia/reoxygenation 1 h,3 h,6 h,12 h group (H/R1 group,H/R3 group,H/R6 group,H/R12 group),4-PBA + H/R6 group.H9C2 cardiomyocytes of control group were cultured under normal condition till the end of experiment.Cardiomyocytes of H/R group underwent hypoxia for 3 hours followed by reoxygenation for 1 h,3 h,6 h,12 h.4PBA were given 2 hours before hypoxia in the 4PBA + H/R6 group.Hoechst 33258 staining was used to observe the apoptosis of cells under fluorescence microscope.The protein expression of Ero1α,glucose-regulated proteins 78 (Grp78),C/EBP homologous protein (CHOP),caspase-12 were detected by Western blot.RESULTS:Compared with the control group,the expression level of Ero1α protein increased with the time of reoxygenation.The expression of Ero1 α increased significantly at 3 h after reoxygenation and reached high point at 6 h (compared with the control group,P ＜ 0.01).Grp78 increased significantly after hypoxia and reoxygenation (compared with the control group,P ＜0.01),reached a peak at 3 h after reoxygenation.The apoptosis rate was also significantly increased,of which the most significant was at reoxygenation 6 h.Take hypoxia 3 h reoxygenation 6 h as the observation point,the expression of Ero1α,GRP78,CHOP and Caspase-12 in H/R6 group was significantly higher than that in control group (P ＜ 0.01).After administration of 4PBA,Ero1α,GRP78,CHOP,Caspase-12 protein expression was significantly lower than that in H/R6 group (P ＜ 0.05,P ＜ 0.01).CONCLUSION:Hypoxia/reoxygenation can induce cardiomyocyte apoptosis and endoplasmic reticulum stress.The expression of Ero1α in cardiomyocytes induced by hypoxia/reoxygenation can be changed.The expression of Ero1α is related to apoptosis.关键词
Ero1α/缺氧复氧/内质网应激/凋亡/心肌细胞Key words
Ero1α/hypoxia/reoxygenation/endoplasmic reticulum stress/apoptosis/cardiomycytes分类
医药卫生引用本文复制引用
刘悦,刘媛媛,张妮,曹释露,张晓京,来丽娜..Ero1α在缺氧复氧致H9C2细胞内质网应激时的表达变化及意义[J].中国临床药理学与治疗学,2017,22(9):972-977,6.基金项目
国家级大学生创新创业训练项目(201610117005),长治医学院创新团队项目(CX201409) （201610117005）
