中国临床药理学与治疗学2017，Vol.22Issue(9)：984-991,8.
基于HPLC-MS/MS的青风藤生物碱的药物代谢动力学研究
HPLC-MS/MS based pharmacokinetic study of alkaloids of sinomenium acutum
摘要
Abstract
AIM:To establish a LC-MS/MS method for the study of pharmacokintic characteristics of alkaloids of sinomenium acutum in sprague dawley rat,and lay the foundation for the extensive research and clinical use of sinomenium acutum.METHODS:(1) LC-MS/MS method for the quantification of alkaloids of sinomenium acutum in biological sample was developed.(2)The in vivo time course of sinomenium acutum extracts (10 g crude drug/kg) in rats after a single oral administration was investigated.Pharmacokinetic parameters were calculated from the concentration data determined by LC-MS/MS.RESULTS:The standard curves showed good linearity over the concentration range of sinoacutine (13.8-3 530.0 ng/mL,R2 =0.999 9) and magnoflorine (5.8-2 960.0 ng/mL,R2 =0.999 8) in rat plasma.The inter-day and intra-day precision and accuracy values of sinoacutine and magnoflorine at three levels of QC samples were less than 15％.Pharmacokinetic study in rats showed that the main parameters for sinoacutine and magnoflorine were Cmax (14.88) and (0.99) μg/mL,Tmax (55.00) and (57.50) min,T1/2 (2.64) and (6.08) h,AUMC(0-t) (369 127.25) and (32 143.44) μg· h · h· mL-1,AUC（0-t） (2 482.76) and (177.77) μg · h-mL-1,MRT(0-t) (2.50) and (3.12) h,respectively.CONCLUSION:A sensitive,specific,and suitable LC-MS/MS method is validated for simultaneously quantifying the concentrations of sinoacutine and magnoflorine in rat plasma.Pharmacokinetic parameters show that the alkaloids of sinomenium acutum in rats conform to the non-compartmental model,and the plasma concentration reaches its peak rapidly,the elimination process is slow with high blood concentration.关键词
青风藤生物碱/LC-MS/MS/药代动力学Key words
alkaloids of sinomenium acutum/LC-MS/MS/pharmacokinetics分类
医药卫生引用本文复制引用
谢彤,程学芳..基于HPLC-MS/MS的青风藤生物碱的药物代谢动力学研究[J].中国临床药理学与治疗学,2017,22(9):984-991,8.基金项目
国家自然科学基金青年科学基金项目(81503138) （81503138）
国家自然科学基金面上项目(81303295) （81303295）
高等学校博士学科点专项科研基金(20133237120001) （20133237120001）
江苏省自然科学基金-青年基金项目(BK20130959) （BK20130959）
