中国临床药理学与治疗学2017，Vol.22Issue(9)：1002-1007,6.
漆黄素对脑缺血再灌注诱导学习记忆损伤及HPA轴的影响
Effect of fisetin on learning and memory impairment induced by cerebral ischemia reperfusion and HPA axis
摘要
Abstract
AIM:To investigate the effect of fisetin on learning memorial ability deficit after cerebral ischemia reperfusion and its possible mechanism.METHODS:Adult male Wistar rats were randomly divided into seven groups:the sham operation,the IR model group,fisetin group(10,20,40 mg/kg,p.o.).The four-vessel occlusion method was used to build rat model of cerebral ischemia reperfusion.All rats were tested for Morris water maze (MWM).The serum level of corticosterone was detected using the enzyme linked immunosorbent assay.At the end,the expressions of corticotropin releasing hormone (CRH) mRNA and glucocorticoid receptor (GR) mRNA in the hippocampus were analyzed by RT-PCR and semi-quantitative in situ hybridization,respectively.RESULTS:Compared with the sham group,the learning ability of the IR rats decreased significantly (P ＜ 0.01),fisetin (20,40 mg/kg) could improve the ability of learning memory significantly(P ＜ 0.05 or P ＜ 0.01).The serum level of corticosterone increased in IR rats,and fisetin (20,40 mg/kg)could also decline the level of this stress hormone (P ＜ 0.05).At the end,the expression of CRH mRNA in the hippocampus of IR rats was increased and fisetin (40 mg/kg)reversed the expression of this gene (P ＜ 0.05);the GR receptor expressions in the hippocampus was reduced and fisetin(40 mg/kg)reversed these changes in IR rats (P ＜ 0.05).CONCLUSION:Fisetin reverses the learning memory damage after cerebral ischemia reperfusion injury,and the mechanism may be mediated by regulating the function of the hypothalamo-pituitary-adrenal (HPA) axis and the expression.关键词
漆黄素/HPA轴/脑缺血再灌注/促肾上腺皮质激素释放激素Key words
fisetin/HPA axis/cerebral ischemia reperfusion/corticotropin releasing hormone分类
医药卫生引用本文复制引用
朱洁瑾,吴湧,叶晓莉,叶华进,王刚,费霏,李晴宇..漆黄素对脑缺血再灌注诱导学习记忆损伤及HPA轴的影响[J].中国临床药理学与治疗学,2017,22(9):1002-1007,6.基金项目
杭州市卫生科技项目(201348758) （201348758）
