中国全科医学2017，Vol.20Issue(29)：3628-3632,5.DOI:10.3969/j.issn.1007-9572.2017.06.y31
白介素5/TNFRSF13B在深部浸润型子宫内膜异位症微环境中的表达及其意义研究
Expression of IL-5 and TNFRSF13B in Deep Infiltrating Endometriotic Milieu and Its Clinical Significance
摘要
Abstract
Objective To investigate the difference in the expressions of IL-5 and TNFRSF13B in deep infiltrating endometriosis (DIE) versus those in the normal endometrium.Methods The enrolled participants were 30 cases who received inpatient treatment in Department of Gynecology,Xiangyang Central Hospital from December 2015 to December 2016,including 15 cases with surgery due to DIE (experimental group),and 15 cases with laparoscopic or open surgery due to fallopian tube recanalization (control group).cDNA microarray,RT-PCR,ELISA,immunohistochemistry and Western blotting were used to detect the expressions of IL-5 and TNFRSF13B in both the DIE and the normal endometrium.Results Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of the cDNA microarray results was performed to screen the genes which expressed in DIE differently from in the normal endometrium,and the results demonstrated that intestinal immune network for IgA production (hsa04672) associated with immune response expressed more highly in DIE,and IL-5 and TNFRSF13B genes in this pathway were chosen for in-depth study.The mRNA expression levels of IL-5 and TNFRSF13B in endometrium epithelium cells (EECs) measured by RT-PCR were much higher in the experimental group than in the control group (P ＜ 0.05).The protein expression levels of IL-5 in both the peritoneal fluid and epithelial cells supernatant detected by ELISA were higher in the experimental group than in the control group (P ＜ 0.05).The protein expression levels of TNFRSF13B in EECs measured by immunohistochemistry was higher in the experimental group than in the control group (P ＜ 0.05).The experimental group had higher protein expression levels of TNFRSF13B in EECs measured by Western blotting than the control group did (P ＜ 0.05).Conclusion Increased expressions of IL-5 and TNFRSF13B in DIE may be associated with the abnormal immune system of DIE patients.The pro-inflammatory and tolerant state of the immune system coexisted in the endometriotic milieu.It can promote the implantation and growth of EECs in abdominal cavity.关键词
子宫内膜异位症/白介素5/跨膜激活剂与CAML交互蛋白质Key words
Endometriosis/Interleukin-5/Transmembrane activator and CAML interactor protein分类
医药卫生引用本文复制引用
杨眉,蒋春樊,哈春芳..白介素5/TNFRSF13B在深部浸润型子宫内膜异位症微环境中的表达及其意义研究[J].中国全科医学,2017,20(29):3628-3632,5.基金项目
国家自然科学基金资助项目(81660251) （81660251）
宁夏科技攻关项目(2016KJHM52) （2016KJHM52）
生殖与遗传基础及临床研究创新团队开放课题(220/2002080102) （220/2002080102）
