中国全科医学2017，Vol.20Issue(30)：3736-3739,4.DOI:10.3969/j.issn.1007-9572.2017.00.087
血浆miR-92a-3p和miR-204-5p及miR-186-5p诊断胆管癌的价值研究
Value of Plasma miR-92a-3p, miR-204-5p and miR-186-5p in the Diagnosis of Cholangiocarcinoma
摘要
Abstract
Background Cholangiocarcinoma (CCA) originates from bile duct epithelial cells.It is occult and highly malignant.The value of circulating miRNA in the diagnosis of CCA remains to be studied.Objective To evaluate the diagnostic value of plasma miR-92a-3p,miR-204-5p and miR-186-5p for CCA,in order to improve the diagnostic level of CAA.Methods Sixteen patients with CCA who met the inclusion criteria and received treatment in the Second Hospital of Hebei Medical University from May 2015 to January 2016 were included in the case group,and other eligible 15 patients without liver and gallbladder diseases treated in the same hospital during the same period were selected as the control group.Reverse transcription-quantitative polymerase chain rection (RT-qPCR) was used to detect the expressions of miR-92a-3p,miR-204-5p and miR-186-5p from plasma samples of all the patients.The Logistic regression equation of miR-92a-3p,miR-204-5p and miR-186-5p was calculated by binary Logistic analysis.Receiver operating characteristic (ROC) curve was drawn to test the diagnostic value of miR-92a-3p,miR-204-5p,miR-186-5p and their combined diagnosis in patients with CCA.The area under the ROC curve (AUC),sensitivity,specificity,and Youden's index were calculated.Results The miR-92a-3p expression in the case group was higher than that in the control group (P ＜ 0.05).However,the expressions of miR-204-5p and miR-186-5p were found to be lower in the case group (P ＜ 0.05).The AUC of miR-92a-3p,miR-204-5p and miR-186-5p for CCA diagnosis was 0.708 [95％ CI (0.524,0.892)],0.725 [95％ CI (0.535,0.915)],0.750 [95％ CI (0.576,0..924)],respectively.The cutoff value of miR-92a-3p,miR-204-5p and miR-186-5p for CCA diagnosis was 0.130 8,0.008 5,0.005 2,respectively.The sensitivity of miR-92a-3p,miR-204-5p and miR-186-5p for CCA diagnosis was 0.938,0.563,0.563,respectively.The specificity of miR-92a-3p,miR-204-5p and miR-186-5p for CCA diagnosis was 0.467,0.933,0.933 respectively.The Youden's index of miR-92a-3p,miR-204-5p and miR-186-5p for CCA diagnosis was 0.405,0.496,0.496 respectively.Binary Logistic analysis showed that Logit (P) =-0.031 + 9.167 × miR-92a-3p-49.453 ×miR-186-5p-66.771×miR-204-5p,the AUC for CCA diagnosis was0.879 [95％CI (0.749,1.010)],and the cutoff value,sensitivity,specificity and Youden's index were 0.095 8,0.938,0.800,and 0.738 respectively.The AUC of miR-92a-3p,miR-204-5p and miR-186-5p for combined diagnosis of CCA was larger than that of miR-92a-3p,miR-204-5p and miR-186-5p for CCA diagnosis alone (P ＜0.05).Conclusion miR-92a-3p,miR-204-5p,and miR-186-5p can be used for the diagnosis of CCA,and their combined diagnosis of CCA is of higher value.关键词
胆管肿瘤/诊断/血浆/肿瘤标记,生物学/微小RNAKey words
Bile duct neoplasms/Diagnosis/Plasma/Tumor markers, biological/MicroRNA分类
医药卫生引用本文复制引用
马俊骥,郭平,郭昱,吴江华..血浆miR-92a-3p和miR-204-5p及miR-186-5p诊断胆管癌的价值研究[J].中国全科医学,2017,20(30):3736-3739,4.基金项目
河北省科技计划项目(14277757D) （14277757D）
