中国全科医学2017，Vol.20Issue(30)：3770-3775,6.DOI:10.3969/j.issn.1007-9572.2017.07.y03
三黄糖肾康颗粒对早期糖尿病肾病模型大鼠微炎症状态的影响
Effect of Sanhuangtangshenkang Granule on the Micro Inflammatory State in Early Diabetes Nephropathy Model Rats
摘要
Abstract
Objective To explore the effect of Sanhuangtangshenkang granule on the micro inflammatory state in rats with early diabetes nephropathy.Methods The study was conducted from August to October in 2012.The study animals were 90 grade SPF Wistar rats,female and male were half and half.10 rats were randomly selected as normal control group and fed with conventional pellet feed.The remaining 80 rats were randomly divided into model group,western nedicine group,traditional Chinese medicine prevention group,traditional Chinese medicine low dose group and traditional Chinese medicine high dose group.There were 16 rats in each group,and they were fed with high fat and high sugar diet.One week later,all the rats in each group were given intraperitoneal injection of streptozotocin (STZ) solution 42 mg/kg,except for the normal control group,in order to induce diabetic model.8 rats failed to make the model;the remaining 72 rats were collected 24 h urine after 2 weeks to detect urinary microalbumin (MALB) to determine whether the early diabetic nephropathy model was successful,among them 6 rats failed to make diabetic nephropathy model.In the course of the experiment,3 rats in the model group died,1 rat in the western medicine group died,2 rats in the traditional Chinese medicine low dose group and high dose group of died,respectively.Both the normal control group and model group were given distilled water by gavage.The rats in traditional Chinese medicine prevention group began the administration at the beginning of the success of early diabetic nephropathy model,and continued for 10 weeks;the other groups were given corresponding medicine by gavage 2 weeks after the model success,and continued for 8 weeks.The western medicine group was given benazepril gavage,the traditional Chinese medicine high dose group used the liquid medicine of 3.84 g/m1 Sanhuangtangshenkang concentration,the traditional Chinese medicine prevention group and the traditional Chinese medicine low dose group used the liquid medicine of 1.28 g/ml Sanhuangtangshenkang concentration.After the experiment,fasting blood glucose (FBG),glycosylated hemoglobin type A1c (HbA1c),microalbuminuria (MALB),β2 microglobulin (β2-MG),serum creatinine (Scr),blood urea nitrogen (BUN),C-reactive protein (CRP) in serum and renal tissues,renal tissue nuclear factor κB (NF-κB),monocyte chemoattractant protein-1 (MCP-1) and cell surface chemokine receptor 2 (CCR2) mRNA and protein expression levels were detected in all rats.The renal pathological morphological changes were observed under light microscope (HE staining and PAS staining).Results The FBG,HbA1c and MALB in model group,western medicine group,traditional Chinese medicine prevention group,low dose group and high dose group were higher than those in normal control group (P ＜ 0.01).The FBG,HbA1c,MALB,CRP in serum and kidney tissues,expression levels of NF-κB,MCP-1 and CCR2 mRNA and protein in kidney tissues in western medicine group,traditional Chinese medicine prevention group,low dose group,high dose group were lower than those in the model group (P ＜ 0.01).The FBG and MALB in traditional Chinese medicine prevention group were lower than those of western medicine group,traditional Chinese medicine low dose group and high dose group (P ＜ 0.05).The levels of BUN in normal control group,western medicine group,traditional Chinese medicine prevention group,low dose group and high dose group were lower than that in model group (P ＜0.01).The BUN in the traditional Chinese medicine low dose group was higher than that in the normal control group (P ＜ 0.05).The CRP in serum and kidney tissues,expression levels of NF-κB,MCP-1 and CCR2 mRNA and protein in kidney tissues in model group were higher than those in normal control group (P ＜ 0.01) The expression levels of NF-κB mRNA and protein in kidney tissues in traditional Chinese medicine low dose group and high dose group were higher than those in traditional Chinese medicine prevention group (P ＜ 0.01).The expression levels of NF-κB mRNA and protein in kidney tissues in western medicine group were higher than those in traditional Chinese medicine prevention group,while lower than those in traditional Chinese medicine low dose group (P ＜ 0.01).The expression levels of MCP-1 and CCR2 mRNA and protein in the rat kidney tissues in traditional Chinese medicine prevention group were significantly lower than those in western medicine group and traditional Chinese medicine low and high dose group (P ＜ 0.05).The expression levels of MCP-1 and CCR2 mRNA and protein in western medicine group were lower than those in traditional Chinese medicine low and high dose group (P ＜ O.05).The renal pathological changes degree of rats in western medicine group,traditional Chinese medicine prevent group,traditional Chinese medicine low and high dose group were significantly lighter than that in the model group.Conclusion Sanhuangtangshenkang granule could effectively reduce FBG,HbA1c,MALB,BUN,CRP in serum and renal tissues of rats with diabetic nephropathy model,down regulate the mRNA and protein expression levels of NF-κB,MCP -1 and CCR2,improve the renal morphological and pathological changes and alleviate the micro inflammatory state of diabetic nephropathy.关键词
糖尿病肾病/大鼠/炎症/三黄糖肾康Key words
Diabetic nephropathies/Rats/Inflammation/Sanhuangtangshenkang granule分类
医药卫生引用本文复制引用
奚悦,李敬林..三黄糖肾康颗粒对早期糖尿病肾病模型大鼠微炎症状态的影响[J].中国全科医学,2017,20(30):3770-3775,6.基金项目
国家自然科学基金资助项目(81173153) （81173153）
