中国全科医学2017，Vol.20Issue(30)：3776-3780,5.DOI:10.3969/j.issn.1007-9572.2017.00.074
穴位埋线对缺血再灌注损伤高脂血症合并2型糖尿病大鼠心肌保护作用的机制研究
Mechanism of Catgut Implantation at Acupoints for the Protection of Myocardium from Ischemia-reperfusion Injury in Hyperlipidemia Rats with Type 2 Diabetes Mellitus
摘要
Abstract
Objective To explore the mechanism of catgut implantation at acupoints for the protection of myocardium from ischemia-reperfusion injury in hyperlipidemia zucker diabetic fatty (ZDF) rats,models of type 2 diabetes mellitus,in order to provide a theoretical basis for clinical treatment.Methods This study was conducted during January 2015 to May 2016.Twenty-four ZDF rats were selected and randomly divided into control group,ischemia reperfusion (IR) group,ischemic pretreatment (IP) group and catgut implantation (CI) group,with 6 in each.On the 7th day after one week of adaptive feeding,myocardial tissues of the control group were taken out via thoracotomy,the other three groups were used for establishing IR models after ligation of the left anterior descending coronary artery.IR group first received myocardial ischemia treatment for 30 min,then received reperfusion for 60 min.IP group received 3 rounds of 5-min myocardial ischemia treatment and 5-min reperfusion,then received 30-min myocardial ischemia treatment and 60-min reperfusion.CI group received the same treatment given to IR group except on the first day after one week of adaptive feeding,they received catgut implantation at Neiguan,Danzhong,and Xinshu acupoints.The ultrastructure and level of autophagy of myocardium of ZDF rats were observed by transmission electron microscope.Western blotting was used to detect the levels of myocardial endoplasmic reticulum stress-related protein glucose-regulated protein 78 (GRP78) and autophagy-related protein Beclin-1.The levels of myeloperoxidase (MPO) and malondialdehyde (MDA) were measured by enzyme-linked immunosorbent assay (ELISA).Results Transmission electron microscope observation showed that,the control group demonstrated normal mitochondria and cardiomyocytes without many autophagysomes;IR group presented significantly swollen mitochondria and cardiomyocytes with ruptured cell membrane and leaked contents,leaving vacuoles or cavities and a small amount of autophagic vacuoles.Compared with IR group,the myocardial cell ultrastructural damage in IP and CI groups was lighter and the number of autophagic vacuoles was significantly increased.The myocardial cell ultrastructural damage in IR group was the most serious,even there were no many autophagic vacuoles.The levels of GRP78,Beclin-1,MPO and MDA were significantly lower in the control group than in the other three groups (P ＜0.05).IR group had lower levels of GRP78 and Beclin-1 but higher levels of MPO and MDA compared with IP and CI groups (P ＜ 0.05).Conclusion Catgut implantation at Neiguan,Danzhong,and Xinshu acupoints may play a role in the protection of myocardium from ischemia-reperfusion injury by increasing the levels of GRP78 and Beclin-1.关键词
再灌注损伤/大鼠/内质网应激/自噬/缺血预处理,心肌/穴位埋线Key words
Reperfusion injury/Rats/Endoplasmic reticulum stress/Autophagy/Ischemic pretreatment, myocardium/Catgut implantation at acupoints分类
医药卫生引用本文复制引用
王一茗,张新昕,束彦页..穴位埋线对缺血再灌注损伤高脂血症合并2型糖尿病大鼠心肌保护作用的机制研究[J].中国全科医学,2017,20(30):3776-3780,5.基金项目
国家自然科学基金青年科学基金项目(81303037) （81303037）
