摘要
Abstract
Background Nuclear factor kappa B (NF-κB) signaling pathway is one of the important inflammatory pathways that is closely related to tumor,and single nucleotide polymorphism (SNPs) are also associated with disease susceptibility.P50/p65 complex is an important molecule in NF-κB signaling pathway of regulating transcription of target genes.Objective To investigate the association of genetic polymorphisms in NF-κB1 and RelA with epithelial ovarian cancer susceptibility among Chinese Han women,so as to provide an experimental basis for the prevention,diagnosis and treatment of epithelial ovarian cancer at molecular level.Methods From February 2014 to February 2015,we selected 286 Chinese Han women with epithelial ovarian cancer who received surgical treatment in Department of Gynaecology of Peking University Third Hospital,Beijing Cancer Hospital,Beijing Hospital from 1999 to 2010 as the case group,and recruited 302 age-matched healthy Chinese Han women underwent physical examination during the same period in the above hospitals as the control group.DNA of the subjects was extracted,SNPs were selected from genotyping data of NF-κB1 and RelA genes of the Chinese Han population in Beijing (CHB) containing all SNPs at the 2 kb neighborhood in the HapMap database.The identified four SNPs in NF-κB1 gene were rs3774934 (A/G),rs3774938 (A/G),rs230510 (A/T),rs909332 (A/T) respectively,and the identified three SNPs in RelA gene were rs10896027 (C/G),rs7119750 (C/T),rs11820062 (C/T) respectively.The SNPs were genotyped by TaqMan probes.Based on the genotyping data,the haplotype block map was constructed.And the haplotype and diplotype of each subject were deduced.Results The rs3774934,rs3774938,rs230510,rs909332,rs10896027,rs7119750 and rs11820062 in the control group met the Hardy-Weinberg equilibrium (P ＞ 0.05).The genotypes and allele frequencies of rs3774934,rs3774938,and rs230510 in NF-κB1 gene did not differ significantly between the two groups (P ＞ 0.05).There was significant difference in genotype of rs909332 in NF-κB1 gene between the two groups (P ＜ 0.05).There was no significant difference in allele frequencies of rs909332 in NF-κB1 gene between the two groups (P ＞ 0.05).Multivariate Logistic regression analysis showed that the genotypes of rs3774934,rs3774938,rs230510 and rs909332 in NF-κB1 gene were not the influencing factors of epithelial ovarian cancer (P ＞ 0.05);haplotypes of NF-κB1 gene were not the influencing factors of epithelial ovarian cancer (P ＞ 0.05);GA-GA diplotype of NF-κB1 gene was an influencing factor of epithelial ovarian cancer (P ＜0.05).There were no significant differences in genotypes and allele frequencies of rs10896027,rs7119750 and rs11820062 in RelA gene between the two groups (P ＞0.05).Multivariate Logistic regression analysis showed that genotypes of rs10896027,rs7119750 and rsl 1820062 in RelA gene were not the influencing factors of epithelial ovarian cancer (P ＞ 0.05);haplotypes of RelA gene were not the influencing factors of epithelial ovarian cancer (P ＞ 0.05);CC-CC diplotype of RelA gene was an influencing factor of epithelial ovarian cancer (P ＜ 0.05).Conclusion Genetic polymorphisms of NF-κB1 and RelA are associated with the susceptibility to epithelial ovarian cancer in Chinese Han women.GA-GA diplotype of rs3774934 (G ＞ A) and rs3774938 (A ＞ G) of NF-κB1 gene and CC-CC diplotype of rs7119750 (C ＞ T) and rs11820062 (C ＞ T) of RelA gene are associated with susceptibility to epithelial ovarian cancer.关键词
卵巢肿瘤/多态性,单核苷酸/汉族/NF-κB/RelAKey words
Ovarian neoplasms/Polymorphism, single nucleotide/Han nationality/NF-kappa B/RelA分类
医药卫生
