中国全科医学2017，Vol.20Issue(35)：4391-4396,6.DOI:10.3969/j.issn.1007-9572.2017.00.098
蝉翼藤单体提取化合物对乙醇诱导小鼠肝脏氧化损伤保护作用研究
Effects of Monomeric Compound Extracted from Securidaca on the Protection of Liver Against Oxidative Damage Induced by Ethanol in Mice
摘要
Abstract
Objective To investigate the protective effect of methyl ferulic acid,a monomeric compound extracted from securidaca based on analyzing its effect on the oxidative damage in liver and the expressions of Bax and Bcl-2 in mice induced by ethanol. Methods From March to June 2017,60 SPF grade male C57BL/ 6 mice were selected and randomized into normal group,model group and low-dose (5 mg/ kg)methyl ferulic acid group,middle-dose (10 mg/ kg)methyl ferulic acid group, high-dose (20 mg/ kg)methyl ferulic acid group,12 in each group. Except the normal group,the other groups were given intragastric administration of 50% ethanol solution in a volume of 0. 1 ml/ 10 g body weight every morning,the low-dose, middle-dose and high-dose methyl ferulic acid groups additionally received intragastric administration of methyl ferulic acid solution in a volume of 5 mg/ kg,10 mg/ kg,20 mg/ kg body weight,respectively every evening,for 4 weeks. Hepatic tissues of the mice were taken out and stained with HE,and their pathological changes were observed. The liver index and splenic index were calculated. Biochemical analyses of serum ALT,AST,TC,TG,catalase (CAT),glutathione peroxidase (GSH-Px), superoxide dismutase (SOD),malondialdehyde (MDA)levels were performed. The expressions of Bax,Bcl-2 mRNA in liver tissues were detected by reverse transcription-polymerase chain reaction (RT-PCR). The expressions of Bax and Bcl-2 in liver tissues were detected by western blotting. Results Increased values of liver index,splenic index,ALT,AST,TC,TG and MDA,elevated expressions of Bax/ Bcl-2 mRNA and Bax/ Bcl-2,and decreased values of CAT,GSH-Px,SOD were found in the model group,low-dose,middle-dose and high-dose methyl ferulic acid groups rather than the normal group (P <0. 05). The model group had higher values of liver index,splenic index,ALT,AST,TC,TG,MDA,increased expressions of Bax/ Bcl-2 mRNA and Bax/ Bcl-2 but lower values of CAT,GSH-Px and SOD than the low-dose,middle-dose and high -dose methyl ferulic acid groups (P < 0. 05). The values of all the above markers differed significantly between the low-dose, middle-dose and high-dose methyl ferulic acid groups (P < 0. 05). Conclusion Methyl ferulic acid can protect the liver against oxidative damage induced by ethanol in mice manifested as improving liver function and alleviating the degree of hepatic injury,and stable the expressions of Bax and Bcl-2,which may be related to its resistance to oxidative damage of liver and inhibition of cell apoptosis.关键词
蝉翼藤属/甲基阿魏酸/乙醇/氧化性应激Key words
Securidaca/Methyl ferulic acid/Ethanol/Oxidative stress分类
医药卫生引用本文复制引用
李辰,黎莹,李丽,杨成芳,钟毓娟,吴丹,石琳,陈莉,李勇文..蝉翼藤单体提取化合物对乙醇诱导小鼠肝脏氧化损伤保护作用研究[J].中国全科医学,2017,20(35):4391-4396,6.基金项目
国家自然科学基金资助项目(81360497) （81360497）
广西高等学校优秀中青年骨干教师培养工程项目 （）
2016年自治区级大学生创新创业训练计划项目(201610601046) （201610601046）
