中国人兽共患病学报2017，Vol.33Issue(11)：951-955,5.DOI:10.3969/j.issn.1002-2694.2017.11.001
不同养殖场家蝇成虫体表携带细菌的分离与鉴定
Bacteria on body surface of Musca domestica adults from different livestock farms
摘要
Abstract
This study aims to isolate and identify the bacteria from the surface of Musca domestica adults in different livestock farms.The adults of Musca domestica were collected with sweep nets,then the surface of adult flies were washed with sterile water,and the eluted bacteria cultured with LB culture medium.The DNA of the bacteria isolated was extracted,16SrDNA fragment was amplified through general PCR,and the 16SrDNA sequence was blasted with bacteria sequence in NC-BI.Results showed that the number of Musca domestica adults and the bacteria from their surface had significant different in different livestock farms.The population density of Musca domestica adults had linear correlation with the number of bacteria from Musca domestica adults and the number of bacteria genus.Among the bacteria isolated from Musca domestica adults,Staphylococcus,Klebsiella,Escherichia and Serratia were the most common genus in the livestock farms.Most of the bacteria isolated from livestock farm house flies were opportunistic human bacterial pathogen,and the risk of bacterial pathogens spread to people was existed.The population density of house flies reflected the management level of different livestock farms.The house flies could mechanical transmit opportunistic human bacterial pathogen from livestock residue to human.So the house flies control and the monitoring of bacteria on body surface of house flies carried should be strengthened.关键词
家蝇/养殖场/体表细菌/机会致病细菌/蝇传疾病Key words
Musca domestica/livestock farms/bacteria on the body surface of house flies/opportunistic bacteria/fly borne diseases分类
医药卫生引用本文复制引用
陈丹,庄桂芬,黄振东,薛志静,刘婧,李妍,张瑞玲,张忠..不同养殖场家蝇成虫体表携带细菌的分离与鉴定[J].中国人兽共患病学报,2017,33(11):951-955,5.基金项目
国家自然科学基金(Nos.81401693,81572028,81271874)和山东省科技发展计划项目(No.2014GSF121007)联合资助 Supported by the National Natural Science Foundation of China (Nos.81401693,81572028 and 81271874),the Science and Technology Development Projects of Shandong Province (No.2014GSF121007) （Nos.81401693,81572028,81271874）
