中国人兽共患病学报2017，Vol.33Issue(11)：972-978,7.DOI:10.3969/j.issn.1002-2694.2017.11.005
29种毒力基因在91株食源性单核细胞增生李斯特氏菌中的分布
Distribution characterization of 29 virulence genes in 91 foodborne Listeria monocytogenes strains
摘要
Abstract
In order to study the distribution of virulence genes of Listeria monocytogenes (Lm) in Hebei Province,29 virulence genes of Lm,including Listeria monocytogenes pathogenicity islands Ⅰ (LIPI-Ⅰ:prfA,plcA,plcB,hlyA,mpl and actA),10 internalins genes (inlA,inlB,inlC,inlD,inlE,inlF,inlG,inlH /C2,inlI and inlJ) and the other 13 virulence-associated genes (bsh,srtA,iap,sigB,virR,mprF,dltA,dltB,dltC,dltD,srtB,fbpA and hpt) were detected by PCR.Results showed that in the 91 Lm strains,the detection rate of 23 virulence genes were 100％.The 29 virulence genes of 26 Lm strains were all detected,and 65 Lm strains had different deletion of 6 virulence genes inlD,inlF,inlG,inlH /C2,inlJ and mpl.The deletion rate of inlG and inlF were 60.44％ and 54.95％,respectively,following by mpl gene,with a deletion rate of 19.78％.According to the absence of virulence genes,91 strains could be divided into 10 subtypes,and the dominant virulence subtypes was type Ⅰ with all 23 virulence genes.The deletion rate of virulent genes in Shijiazhuang was higher than that in northern Hebei.It is suggested that the rate of virulence gene of food-borne Lm in Hebei Province is high,and the virulence gene deletion patterns has diversity and regional differences.关键词
单核细胞增生李斯特菌/毒力基因/分布特征Key words
Listeria monocytogenes/virulence genes/distribution characteristics分类
医药卫生引用本文复制引用
李秀娟,赵冬,潘琢,高伟利,徐保红,秦丽云..29种毒力基因在91株食源性单核细胞增生李斯特氏菌中的分布[J].中国人兽共患病学报,2017,33(11):972-978,7.基金项目
河北省医学科学研究重点课题(No.ZL20140244)Supported by the Hebei Medical Scientific Research Fund Project(No.ZL20140244) （No.ZL20140244）
