中国人兽共患病学报2017，Vol.33Issue(11)：984-990,7.DOI:10.3969/j.issn.1002-2694.2017.11.007
不同动物源肠球菌耐药性及毒力基因测定与分析
Drug resistance spectrum and virulence gene distribution in Enterococcus isolates of animal origin
摘要
Abstract
One hundred and twenty enterococcal isolates,which were identified from animal specimen,were investigated for the drug-resistance and virulence gene status of Enterococci,so as to provide reference for clinical therapy as well as exploration of Enterococcus pathogenicity.For the 120 Enterococcus isolates,six virulence genes cylA,efaA,Gele,esp,ace and AS were detected with PCR method,and drug-resistance of 11 commonly used antibiotics were measured by K-B disk diffusion tests.The results showed that all of the 120 isolates presented resistance to at least 3 kinds of drugs,and multi-resistance occurred among different originated strains,especially prominent among those isolates from dead swine organs.Generally,the isolates performed highest resistance ratio against lincomycin (115/120),and more than 50 percent isolates were resisted to ciprofloxacin,kanamycin,tetracycline and dalfopristin,while the isolates were least resistant to teicoplanin (1/120).The incidence of cylA,efaA,GelE,esp,ace and AS virulence genes,as a whole,was 39.17％,56.67％,68.33％,23.33％,26.67％ and 13.33％,respectively,but it varied markedly among those of different sources,which showed consistent trend with that drug resistance.It is concluded that the drug-resistance incidence among the test enterococcal isolates showed correlation with that of virulence gene status,and both were closely related to the bacteria origin,therefore,the conventional opportunistic Enterococcus is of value for further exploring these profound relations.关键词
动物/肠球菌/毒力基因/耐药性/聚合酶链式反应Key words
animal/Enterococcus/virulence gene/drug-resistance/polymerase chain reaction分类
医药卫生引用本文复制引用
陈丽颖,张留君,秦明明,陈飞,金钺,胡慧,王亚宾..不同动物源肠球菌耐药性及毒力基因测定与分析[J].中国人兽共患病学报,2017,33(11):984-990,7.基金项目
河南省重点科技攻关项目(No.152102310081)Supported by the Henan Provincial Science and Technology Key Project (No.152102310081) （No.152102310081）
