中国输血杂志2017，Vol.30Issue(10)：1146-1149,4.DOI:10.13303/j.cjbt.issn.1004-549x.2017.10.017
筛选试剂红细胞的血型基因分型方法建立和应用
Establishment and application of blood group genotyping methods for screening antigens on reagent red cells
摘要
Abstract
Objective To establish genotyping methods for important blood groups and screen antigens on reagent red cells.Methods Polymerase chain reaction-sequence specific primers (PCR-SSP) genotyping methods were established for initial analysis important red blood systems (RHCE,Kidd,MNS,Duffy,Diego,Lutheran,Colton,Kell blood systems).As for Diego and Colton blood systems,genotyping was achieved by direct sequencing methods due to unavailability of corresponding reagent antibody.In the other 6 blood systems,Genotyping was validated using serologic typing.82 immobilized O blood donors were genotyped for the eight blood systems by the two methods mentioned prior.Results The genotyping results were consistent with serological and sequencing results.Samples from 13 out of the 82 immobilized O blood donors were con sidered reagent-ready red cells.Conclusion This study has successfully established an accurate,convenient and low-cost PCR-SSP genotyping method,which can screen out reagent red cells from a large number of blood donors with a sensible an tigen panel design.关键词
红细胞血型/试剂红细胞/PCR-SSP基因分型/抗原谱Key words
red blood groups/reagent red cells/PCR-SSP genotyping/antigen panel分类
临床医学引用本文复制引用
徐弘,孔玉洁,宋宁,肖洁,刘忠,田力..筛选试剂红细胞的血型基因分型方法建立和应用[J].中国输血杂志,2017,30(10):1146-1149,4.基金项目
四川省科技厅科技计划项目(2015JY0121) （2015JY0121）
