中国实验动物学报2017，Vol.25Issue(6)：600-604,5.DOI:10.3969/j.issn.1005-4847.2017.06.003
不同感染途径致大鼠肺炎模型制备的比较
Comparison on the preparation of rat models of pneumonia by different infection route
摘要
Abstract
Objective To explore the preparation of a rat model of pneumonia model induced by Pseudomonas aeruginosa( PA) using different methods,and to lay the foundation for further studies. Methods 48 SD rats were randomly divided into 4 groups:the control group (A), the intratracheal injection group (B), the trachea cannulation group (C) and the intranasal inoculation group ( D) . After intervention with different treatment modalities, the body weight,tempera-ture,white blood cell count and lung pathological changes in the rats of all groups were detected at 5, 10, 15 days. Results 1. The behavior, body weight, temperature, leukocytes and pathological inflammatory changes of the lung in rats of the model groups were significantly different from that of control group. 2. Pseudomonas aeruginosa was detected in rats of all the model groups, but the control group was negative. Conclusions Rat model of Pseudomonas aeruginosa infected pneu?monia can be successfully established by intranasal inoculation. This method can avoid the inflammatory interference from operation, and is simple and suitable for popularization.关键词
铜绿假单胞菌/耐药菌/肺炎/炎症反应/大鼠Key words
Pseudomonas aeruginosa/Drug resistant bacteria/Pneumonia/Inflammatory reaction/Rats分类
生物科学引用本文复制引用
丁军颖,郭玉红,刘清泉,高翔,洪燕英,桂红,韦志友,丁雪霏,卢幼然,安世栋,崔煦然..不同感染途径致大鼠肺炎模型制备的比较[J].中国实验动物学报,2017,25(6):600-604,5.基金项目
国家自然科学基金项目(No.81503399,No.81373813) （No.81503399,No.81373813）
国家"重大新药创制"科技重大专项(No.2013ZX09102026) （No.2013ZX09102026）
首都医科大学附属北京中医医院院级课题(No.YJ-201734) （No.YJ-201734）
首都医科大学基础-临床科研合作基金(No.16JL74). （No.16JL74）
