中国实验动物学报2017，Vol.25Issue(6)：611-617,7.DOI:10.3969/j.issn.1005-4847.2017.06.005
高脂饮食对西藏小型猪胰岛素抵抗及肝胰岛素受体底物1、2表达的影响
Influence of high fat diet on insulin resistance and expression of liver insulin receptor substrate 1 and 2 in Tibet minipigs
摘要
Abstract
Objective To explore the effects of high fat diet on insulin resistance ( IR) and the expression of liver insulin receptor substrate ( IRS) 1 and 2 in Tibet minipigs. Methods Ten Tibet minipigs were randomly divided into 2 groups, normal control (Ctr, n=5) group was fed with normal diet, and IR model (n=5) group fed with high fat/choles-terol diet for 12 weeks. After the establishment of pig models for 12 weeks, the body weight and body length were measured and body mass index ( BMI) was calculated, and the changes of total cholesterol ( TC) , low density lipoprotein ( LDL?C) , high density lipoprotein ( HDL?C) , triglyceride ( TG) , free fatty acids ( FFA) , fasting blood glucose ( FBG) , fasting insu?lin ( insulin) and homeostasis model assessment?insulin resistance ( HOMA?IR) were detected. Glucose tolerance test was performed, the area under the curve of glucose tolerance ( AUC) was also calculated, and the expressions of IRS?1 and IRS?2 gene and protein in liver tissue were detected. The lipid deposition, liver glycogen and pathological changes were ex?amined by pathology using oil?red O, PAS and HE staining, respectively. Results Compared with the control group, the body weight, BMI index, TC, LDL?C, HDL?C, FFA, FBG, insulin and HOMA?IR were significantly increased ( P <0. 05, P<0. 01). Intravenous glucose tolerance test showed that the curve of blood glucose and insulin levels were slowed down, while AUCglucose and AUCinsulin were significantly increased (P<0. 05, P<0. 01). Lipid deposition and liver glyco?gen were increased, and partial hepatocyte swelling, part of the nuclei disappeared or were pushed to one end, occasionally scattered infiltration of lymphocytes in the liver tissue. Furthermore, the expressions of IRS?1 and IRS?2 mRNA and protein were significantly decreased (P<0. 05, P<0. 01). Conclusions High fat diet can induce insulin resistance in Tibet minipigs. The decreased IRS?1 and IRS?2 expression in the liver may be one of the molecular mechanisms involved in the effects of high fat diet on insulin sensitivity in Tibet minipigs.关键词
西藏小型猪/高脂饮食/胰岛素抵抗/胰岛素受体底物Key words
Tibet minipig/High fat diet/Insulin resistance/Insulin receptor substrate分类
生物科学引用本文复制引用
潘永明,戎亦骊,黄俊杰,徐孝平,陈诚,陈民利..高脂饮食对西藏小型猪胰岛素抵抗及肝胰岛素受体底物1、2表达的影响[J].中国实验动物学报,2017,25(6):611-617,7.基金项目
浙江省科技厅公益性项目(No.2015C37106) （No.2015C37106）
国家自然科学基金项目(No.31572346) （No.31572346）
浙江省自然科学基金项目(No.LY16C040001) （No.LY16C040001）
浙江中医药大学校级科研基金项目(No.2015ZG19) （No.2015ZG19）
浙江中医药大学比较医学创新团队(No.XTD201301). （No.XTD201301）
