中国实验动物学报2017，Vol.25Issue(6)：624-631,8.DOI:10.3969/j.issn.1005-4847.2017.06.007
长爪沙鼠体外受精与早期胚胎体外培养体系的初步建立
Establishment of a modified in vitro mongolian gerbil oocyte fertilization and embryo culture system
摘要
Abstract
Objective To investigate the feasibility of self-made capacitation liquid for in vitro fertilization of Mongolian gerbils, and to provide a reference for gerbil embryo cryopreservation. Methods In vitro fertilization of Mongo?lian gerbil was performed with the self?prepared capacitation solution and semen, and the 2?cell embryos of Mongolian ger?bils were cultured in vitro using an improved KSOM culture medium. Results The in vitro fertilization rate of gerbils was over 60%, and some gerbil 2?cell embryos could develop further in vitro. Conclusions An in vitro fertilization and embry?o development system of Mongolian gerbil has been established, but it needs further optimization.关键词
长爪沙鼠/体外受精/体外培养Key words
Mongolian gerbil/In vitro fertilization/In vitro culture分类
生物科学引用本文复制引用
郭红刚,萨晓婴,应华忠,褚晓峰,李莉,周生来,卢领群,李坤,杜江涛,石巧娟,金秀清,李长龙..长爪沙鼠体外受精与早期胚胎体外培养体系的初步建立[J].中国实验动物学报,2017,25(6):624-631,8.基金项目
国家科技支撑计划子课题(No.2015BA109B01-02) （No.2015BA109B01-02）
浙江省级公益性技术应用研究计划(No.2015C37119) （No.2015C37119）
浙江省卫生厅课题(No.2015KYB093). （No.2015KYB093）
