中国兽医科学2017，Vol.47Issue(12)：1497-1502,6.DOI:10.16656/j.issn.1673-4696.2017.12.006
猪繁殖与呼吸综合征病毒抗体的荧光微球免疫学检测方法的建立
Development of a fluorescent microbead-based immunoassay for the detection of porcine reproductive and respiratory syndrome virus IgG
摘要
Abstract
In order to establish a high-throughput,reproducibleand highly specific method to detect swine reproductive and respiratory syndrome virus(PRRSV) antibodies,the purified non-structural protein 7 (Nsp7) recombinant protein was used as an antigen to establish a fluorescent microsphere-based immunoassay(FMIA).SDS-PAGEand Western-blot analysis showed that PRRSV Nsp7 recombinant was soluble and had good antigenicity.The intra-assay and inter-assay coefficient of variation ofMFIA was 3.2％ and 4.2％,respectively,and there were no cross-reactions with the positive sera of other swine diseases.When the concentration of mAb was as low as 1 ng/mL,the method still had high sensitivity.These results indicate that the FMIA is rapid,sensitive and specific for detecting antibodies against swine PRRSV.关键词
猪繁殖与呼吸综合征病毒/非结构蛋白7/原核表达/荧光微球免疫学检测方法Key words
PRRSV/Nsp7 protein/prokaryotic expression/fluorescent microsphere-based immunoassay分类
农业科技引用本文复制引用
曾梦,纪方晓,冀池海,陈万里,张桂红,王衡..猪繁殖与呼吸综合征病毒抗体的荧光微球免疫学检测方法的建立[J].中国兽医科学,2017,47(12):1497-1502,6.基金项目
国家重点研发计划项目(2016YFD0500707) （2016YFD0500707）
广东省高等学校优秀青年教师培养计划项目(YQ2015030) （YQ2015030）
