中国兽医科学2017，Vol.47Issue(12)：1528-1532,5.DOI:10.16656/j.issn.1673-4696.2017.12.010
H3N2犬流感病毒PB2 D701N位点突变对小鼠致病性的影响
Effects of PB2 D701N mutation on the pathogenicity of H3N2 canine influenza virus in mice
摘要
Abstract
In order to explore the effect of PB2 D701N mutation on the pathogenicity of H3N2 canine influenza virus (CIV),we rescued two viruses (wild-type virus:rC/GD/02,mutation virus:rC/GD/02-701N) by reverse genetic system and site-directed mutagenesis technology.The polymerase activity assay showed that the polymerase activity of the mutant virus was significantly stronger than that of the wild-type virus(P＜0.05),indicating PB2 D701N mutation could significantly enhance the polymerase activity of H3N2 CIV.The virus growth curve showed that,comparing wild-type virus,the virus replication ability of mutation virus was decreased slightly in MDCK cells.The mice experiment showed that the two strains resulted in a slight decrease in the weight gain rate in mice,but there was no significant difference between two groups and did not cause the mice to die,indicating that PB2 D701N mutation has no significant effects on the pathogenicity of CIV H3N2 in mice.关键词
犬流感病毒/H3N2/定点突变/反向遗传/D701NKey words
canine influenza virus/H3N2/site-directed mutagenesis/reverse genetic/D701N分类
农业科技引用本文复制引用
曾伟杰,黄三,张欣,王丽芳,郑运,周沛,李守军..H3N2犬流感病毒PB2 D701N位点突变对小鼠致病性的影响[J].中国兽医科学,2017,47(12):1528-1532,5.基金项目
国家自然科学基金项目(31372448,31672563) （31372448,31672563）
国家重点研发计划项目(2016YFD0501004) （2016YFD0501004）
公益性行业(农业)科研专项(201303042) （农业）
广东省促进科技服务业发展计划项目(2013B040200032) （2013B040200032）
广东省兽医临床重大疾病综合防控重点实验室项目(2013A061401013) （2013A061401013）
