中国兽医科学2017，Vol.47Issue(12)：1562-1569,8.DOI:10.16656/j.issn.1673-4696.2017.12.015
福建猫源弓形虫IgG抗体检测及PCR-RFLP基因分型与遗传变异分析
Detection of antibody IgG, PCR-RFLP genotyping and sequence variation analysis of Toxoplasma gondii from cat in Fujian
摘要
Abstract
The IgG against Toxoplasma gondii was detected for 40 cat blood samples from a pet market in Fujian using ELISA method and then T.gondii DNA from the positive blood samples was tested by PCR using T.gondii B1 gene primers.The genetic diversity of T.gondii DNA was typed at 12 gentic markers using the multilocus PCR-RFLP,including nuclear loci SAG1,5'SAG2,3'SAG2,alternative SAG2,SAG3,BTUB,GRA6,c22-8,c29-2,L358,PK1 and an apicoplast lucus Apico.The sequences of SAG1,SAG2 and SAG3 were used for variation analysis.The results showed that the positive rate of IgG against T.gondii was 47.5％(19/40) in cat blood samples.Three T.gondii B1 gene DNA was detected by PCR amplification in these 19 positive blood samples.The multilocus PCR-RFLP results and the sequences of 3 loci were analyzed and compared with reference strains RH(Type Ⅰ),PRU(Type Ⅱ),and the result revealed the 3 T.gondii isolates inFujian were found tobe anunique genotypeandgrouped toanewgenotype in this study.关键词
猫/弓形虫/血清学/基因分型/遗传变异Key words
cat/Toxoplasma gondii/serology/genotyping/variation analysis分类
农业科技引用本文复制引用
黄翠琴,陈仁锋,王琨,温福利,郑新添,王寿昆..福建猫源弓形虫IgG抗体检测及PCR-RFLP基因分型与遗传变异分析[J].中国兽医科学,2017,47(12):1562-1569,8.基金项目
福建省科技厅省属高校科研项目(Jk2011052) （Jk2011052）
龙岩市科技计划项目(2012LY45) （2012LY45）
