中国实用口腔科杂志2017，Vol.10Issue(11)：670-673,4.DOI:10.19538/j.kq.2017.11.007
槟榔碱对Hacat及DOK细胞FHIT和SMAD4蛋白表达影响研究
Study on the expression of the FHIT and SMAD4 in Hacat and dysplastic oral keratinocyte cells under the stimulation of arecoline
摘要
Abstract
Objective To explore the possible pathogenesis of oral potentially malignant disorders,we investigated the effect of arecoline on human normal keratinocyte Hacat cells and dysplastic oral keratinocyte with respect to cell survival rate and the expression of the FHIT and SMAD4.Methods Human keratinocyte cells (of the Hacat cell line)and dysplastic oral keratinocytes were treated with different concentrations (0,2.5,5,10,20,40,80 and 160 μg/mL)and different effecting time (24,48,72 h)of arecoline,following which cell survival rate was accessed using the MTI assay.Western blot analysis was performed to study the expression of the FHIT and SMAD4 when Hacat and dysplastic oral keratinocytes were treated with different concentrations (0,20,40,60 and 80 μg/mL)of arecoline for 24 hours.Results MTI assay showed that the survival rate of both Hacat and dysplastic oral keratinocytes were significantly decreased with the increasing concentration and the extension of the effecting time of arecoline.Furthermore,higher survival rate was found in dysplastic oral keratinocyte when compared with Hacat cells under the same stimulation (P ＜ 0.05).The initial expression of FHIT and SMAD4 was higher in Hacat cells than that in dysplastic oral keratinocyte (P ＜ 0.05).Both of these two cell lines had a decreasing expression of FHIT and SMAD4 after being treated with increasing concentration of arecoline for 24 hours.Conclusion The cell survival rate is negatively associated with the arecoline in a dose and time-dependent manner.The expression of FHIT and SMAD4 is also reduced with the increasing concentration of arecoline.Furthermore,the aberrant expression of FHIT and SMAD4 may play an important role in the modulating of cell proliferation and thus may participate in the occurrence and development of oral potentially malignant disorders.关键词
Hacat细胞/异常增生角质形成细胞/槟榔碱/FHIT蛋白/SMAD4蛋白Key words
Hacat cell/dysplastic oral keratinocyte, DOK/arecoline/FHIT/SMAD4分类
医药卫生引用本文复制引用
夏璐璐,高义军,尹晓敏..槟榔碱对Hacat及DOK细胞FHIT和SMAD4蛋白表达影响研究[J].中国实用口腔科杂志,2017,10(11):670-673,4.基金项目
湖南省财政厅资助项目(湘财教指[2015]199号) （湘财教指[2015]199号）
