中国现代医学杂志2018，Vol.28Issue(1)：6-10,5.DOI:10.3969/j.issn.1005-8982.2018.01.002
亲和吸附材料应用于血液灌流治疗外源性内毒素血症的安全性研究
Safety of affinity adsorption material used in hemoperfusion in treatment of exogenous endotoxemia
摘要
Abstract
Objective To evaluate the safety of affinity adsorption material for the removal of exogenous endotoxin. Methods Fifteen beagle dogs were randomly divided into treatment group (n = 10) and control group (n = 5) after intravenous infusion of endotoxin. The treatment group was treated with a blood perfusion apparatus developed by our team, while the control group did not use it. Vital signs were monitored before and after the experiment. Blood samples were collected to detect the biochemical indexes, blood routine and coagulation function at the beginning of perfusion, after perfusion for 60 and 120 min. Results After treatment, the body temperature and respiratory rate of the treatment group gradually decreased, while those of the control group increased. At the beginning of perfusion and 120 min of perfusion, PLT was (222.50 ± 14.19) × 109/L and (199.00 ± 18.18) × 109/L in the treatment group, and (233.91 ± 12.22) × 109/L and (222.07 ± 7.62) × 109/L in the control group, there were significant differences between the two groups (P < 0.05); WBC was (7.14 ± 0.10) × 109/L and (6.10 ± 0.19) × 109/L in the treatment group, and (7.17 ± 0.11) × 109/L and (5.52 ± 0.54) × 109/L in the control group, there were significant differences between the two groups (P < 0.05). Liver function indexes including ALT, AST, ALP, TP and GLO of the treatment group at 120 min of perfusion were significantly lower than those at the beginning of perfusion (P < 0.05), while the indexes of the control group at 120 min of perfusion were higher than those at the beginning of perfusion (P < 0.05). Renal function indices including urea, creatinine and uric acid also showed downward trends after perfusion for 120 min in the treatment group, while increased slightly in the control group. Conclusions It is safe to remove the endotoxin in the blood of the dog by affinity adsorption material.关键词
亲和吸附材料/血液灌流/比格犬/内毒素血症/安全性Key words
affinity adsorption material/hemoperfusion/beagle dog/endotoxemia/safety分类
医药卫生引用本文复制引用
向勇平,崔辉,于杰,刘丹,刘理金,周立冬,杨建明,刘爱兵..亲和吸附材料应用于血液灌流治疗外源性内毒素血症的安全性研究[J].中国现代医学杂志,2018,28(1):6-10,5.基金项目
国家高技术研究发展计划(No:2015AA033904) （No:2015AA033904）
