中国现代医学杂志2018，Vol.28Issue(1)：16-20,5.DOI:10.3969/j.issn.1005-8982.2018.01.004
胃袖状切除术改善2型糖尿病小鼠脂肪组织胰岛素抵抗机制研究
Effect of sleeve gastrectomy on insulin resistance in adipose tissue of obesity-associated type 2 diabetes (+db/+db) mice
摘要
Abstract
Objective To observe the effect of sleeve gastrectomy (SG) on the expression of caveolin-1 in adipose tissue and insulin resistance status. Methods Thirty 10-week old obesity-associated type 2 diabetes (+db/+db) (C57BL/KsJ) mice were randomly allocated to db/db-SG group, db/db-sham group and db/db blank control group, each group had 10 rats. Body weight and food intake were recorded 3 d before operation and 7, 14, 21 and 28 d after operation. On the 5th d before operation and the 30th d after operation, FBG and OGTT were detected before and after operation; ELISA was used to detect the plasma insulin, TG, TC and LDL; RT-PCR and Western blot were used to detect the expressions of caveolin-1 and GLUT4 in the viceral adipose tissue. Results Compared with the db/db-sham group and the blank control group, the mice in the SG group showed obviously-reduced body weight and food intake (P < 0.05), apparent downward trends of FBG level, AUC of OGTT, and HOMA-IR (P < 0.05). The plasma TC, TG and LDL levels in the SG group decreased as well (P < 0.05). The expressions of caveolin-1 and GLUT4 protein and mRNA in the viceral adipose tissue significantly increased in the SG group compared to the db/db-sham group and the blank control group (P < 0.05). Conclusions SG can significantly lower the body weight and food intake, decrease the plasma TC, TG and LDL, and alleviate the insulin resistance status in a short period after surgery, which may be related to reduced expression of caveolin-1 and GLUT4 in the adipose tissue.关键词
袖状胃切除/脂肪组织/胰岛素抵抗/脂肪组织微囊蛋白1Key words
gastric sleeve surgery/adipose tissue/insulin resisitance/caveolin-1分类
医药卫生引用本文复制引用
赵文嫣,孙明,冯勇,耿东华,刘金钢..胃袖状切除术改善2型糖尿病小鼠脂肪组织胰岛素抵抗机制研究[J].中国现代医学杂志,2018,28(1):16-20,5.基金项目
沈阳市科学技术计划项目应用基础研究专项(No:150422) （No:150422）
辽宁省自然科学基金(No:2015020505) （No:2015020505）
