中国现代医学杂志2018，Vol.28Issue(1)：25-29,5.DOI:10.3969/j.issn.1005-8982.2018.01.006
羟基喜树碱对人肺癌A549细胞自噬的影响
Effect of hydroxycamptothecin on autophagy of A549 lung cancer cells
摘要
Abstract
Objective To investigate whether autophagy is involved in the anticancer mechanisms of hydroxycamptothecin (HCPT) in human lung cancer A549 cells in vitro. Methods Human non-small cell lung cancer cell line A549 was cultured. In our study, cell viability was assessed by MTT assay, Cyto-ID staining was used for autophagy detection which was observed by laser scanning confocal microscope (LSCM), the expression of LC3II/I and p-mTOR were measured by Western blot. Results HCPT (50, 200 and 400 μmol) suppressed the viability of A549 cells in a concentration-dependent manner. Moreover, HCPT increased autophagy level in the A549 cells. The autophagy inhibitor 3-MA caused a statistically significant increase in growth inhibition of the HCPT-treated A549 cells. Conclusions HCPT induces autophagy in lung cancer A549 cells in vitro. HCPT (50, 200 and 400 μmol) suppresses the viability of A549 cells in a concentration-dependent manner and its induction of cell autophagy is related to the dosage. Autophagy inhibition combined with HCPT may be a better way for the treatment of lung cancer.关键词
肺癌/自噬/羟基喜树碱/3-MA/mTORKey words
lung cancer/autophagy/HCPT/3-MA/mTOR分类
医药卫生引用本文复制引用
魏艳杰,田炜,王宏丽,彭子荷,李晨浩,郝帅林,张媛,李朝阳,肖童,郝小惠,刘和亮..羟基喜树碱对人肺癌A549细胞自噬的影响[J].中国现代医学杂志,2018,28(1):25-29,5.基金项目
河北省高等学校科学技术研究重点项目(No:ZD2017063) （No:ZD2017063）
唐山市国际科技合作项目(No:14160201B) （No:14160201B）
2016年地方高校国家级大学生创新创业训练计划项目(No:201610081026) （No:201610081026）
华北理工大学大学生创新实践计划项目(No:X2016026) （No:X2016026）
