中国现代医学杂志2018，Vol.28Issue(1)：30-36,7.DOI:10.3969/j.issn.1005-8982.2018.01.007
α-倒捻子素对MCF-7乳腺癌增殖、生长和侵袭的影响及其作用机制
Effect of α-mangostin on proliferation, growth and invasion of MCF-7 breast cancer and its mechanisms
马林伟 1徐红涛 1戴小丽 1韩中保1
作者信息
- 1. 盐城卫生职业技术学院 临床医学教研室,江苏 盐城 224005
- 折叠
摘要
Abstract
Objective To explore the effect of α-mangostin on proliferation, growth, and invasion of MCF-7 breast cancer and its mechanisms. Methods MTS was used to study the effect of α-mangostin on in vitro proliferation of MCF-7 breast cancer cells. MCF-7 breast cancer xenograft model was used to examine the effect ofα-mangostin on in vivo growth of the tumor. The effect of α-mangostin on invasion ability of MCF-7 breast cancer cells was examined by Transwell experiment. Immunohistochemistry was used to study the effect of α-mangostin on the expressions of translationally-controlled tumor protein (TCTP), matrix metalloproteinase-2 (MMP-2) and MMP-9 in MCF-7 subcutaneous transplantation tumor tissue. Western blot was used to detect the expressions of Erk, p38, MMP-2 and MMP-9 proteins. Results The proliferation of MCF-7 breast cancer cells was significant inhibited byα-mangostin in vitro compared to the control group. α-mangostin significantly inhibited the growth of MCF-7 breast cancer subcutaneous transplantation tumor in vivo compared to the control group. The invasion ability of MCF-7 breast cancer cells was significantly inhibited by α-mangostin. The expressions of TCTP, MMP-2 and MMP-9 were significantly inhibited by α-mangostin as shown in immunohistochemistry. The results of Western blot showed that α-mangostin inhibited the expressions of MMP-2, MMP-9, p-Erk and p-p38. Conclusions α-mangostin has anti-proliferation, anti-growth and anti-invasion effect on MCF-7 breast cancer, and such effect can attribute to its inhibition of growth and invasion-related proteins in MCF-7 breast cancer.关键词
α-倒捻子素/乳腺癌/增殖/生长/侵袭/作用机制Key words
α-mangostin/breast cancer/proliferation/growth/invasion/action mechanism分类
医药卫生引用本文复制引用
马林伟,徐红涛,戴小丽,韩中保..α-倒捻子素对MCF-7乳腺癌增殖、生长和侵袭的影响及其作用机制[J].中国现代医学杂志,2018,28(1):30-36,7.
