中国现代医学杂志2018，Vol.28Issue(1)：37-43,7.DOI:10.3969/j.issn.1005-8982.2018.01.008
结肠癌血清microRNA表达谱的检测及临床应用价值
Detection of serum microRNA profile in colon cancer and its clinical application value
摘要
Abstract
Objective To explore the potential novel serum microRNA (miRNA) profile in colon cancer and discuss its clinical application value. Methods The difference of serum miRNA expression between colon cancer patients and healthy people was detected by miRNA microarray chip technology. The differential expression of miRNAs in serum of 60 cases of colon cancer and 60 normal people were validated by qRT-PCR. Results A total of 87 miRNAs were differentially expressed in the serum of the colon cancer patients compared to the normal people, among which 39 miRNAs were upregulated and 48 miRNAs were down-regulated. miR-31, miR-141, miR-224-3p, miR-576-5p and miR-4669 expressions in the colon cancer patients were significantly different from those in the normal control group (P < 0.05). MicroRNA profile (including miR-31, miR-141, miR-224-3p, miR-576-5p and miR-4669) has a higher efficiency in diagnosis of colon cancer (area under the ROC curve was 0.992). Conclusions Clinical detection of expression profile of circulating molecular markers miR-31, miR-141, miR-224-3p, miR-576-5p and miR-4669 in colon cancer patients is useful for the diagnosis of colon cancer. It is expected to be an important index for evaluation of clinical diagnosis and treatment in patients with colon cancer.关键词
结肠癌/血清microRNA/miRNAKey words
colon cancer/serum microRNA/expression profile分类
医药卫生引用本文复制引用
王亚南,陈昭华,陈卫昌..结肠癌血清microRNA表达谱的检测及临床应用价值[J].中国现代医学杂志,2018,28(1):37-43,7.基金项目
苏州市"科教兴卫"青年科技项目(No:KJXW2014017) （No:KJXW2014017）
